Faculty of New Technologies and Engineering, Shahid Beheshti University, Tehran, Iran.
Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Colloids Surf B Biointerfaces. 2019 Jul 1;179:270-279. doi: 10.1016/j.colsurfb.2019.03.054. Epub 2019 Mar 27.
In this study, in situ glyoxal crosslinked chitosan/poly (vinyl alcohol) (PVA) hydrogel nanofibers reinforced with halloysite nanotubes (HNT) were prepared by the electrospinning method without needing post-treatment for stabilization of the nanofibers in aqueous media. FTIR spectroscopy approved the formation of acetal bonds between glyoxal and hydroxyl groups of PVA and chitosan. Morphological studies by SEM/EDX and TEM in accordance with XRD patterns proved that HNT was successfully incorporated into the crosslinked chitosan/PVA nanofibers. The crosslinked nanofibers were insoluble in water. Due to the hydrophilic nature of HNT, the swelling of the nanofibers was increased from 272% for crosslinked chitosan/PVA nanofibers to around 400% for the HNT reinforced nanocomposite nanofibers. Comparing to chitosan/PVA nanofibers, the tensile strength of the crosslinked nanocomposite nanofibers was increased to 2.4 and 3.5 fold by incorporation of 3 and 5% HNT, respectively. Presence of HNT in chitosan/PVA nanofibers reduced the contact angle with water and increased the hydrophilicity of HNT-reinforced nanofibers favoring the attachment of fibroblast cells. Cytotoxicity studies by AlamarBlue assay showed that presence of HNT increased the biocompatibility of the nanofibers. It was also concluded that glyoxal can be used safely for crosslinking of chitosan/PVA nanofibers without any cytotoxic effect for fibroblast cells. From the results of this work, HNT reinforced chitosan/PVA nanofibers crosslinked by glyoxal are introduced as promising nanomaterials for skin tissue regeneration.
在这项研究中，通过静电纺丝法制备了原位交联壳聚糖/聚乙烯醇（PVA）水凝胶纳米纤维，其中加入了埃洛石纳米管（HNT）作为增强材料，而无需对纳米纤维进行后处理以稳定在水介质中。傅里叶变换红外光谱（FTIR）证实了乙二醛和 PVA 及壳聚糖上的羟基之间形成了缩醛键。SEM/EDX 和 TEM 结合 XRD 图谱的形态学研究证明，HNT 已成功掺入交联壳聚糖/PVA 纳米纤维中。交联纳米纤维不溶于水。由于 HNT 的亲水性，纳米纤维的溶胀从交联壳聚糖/PVA 纳米纤维的 272%增加到 HNT 增强纳米复合材料纳米纤维的约 400%。与壳聚糖/PVA 纳米纤维相比，通过掺入 3%和 5%的 HNT，交联纳米复合材料纳米纤维的拉伸强度分别增加到 2.4 倍和 3.5 倍。HNT 存在于壳聚糖/PVA 纳米纤维中降低了与水的接触角，增加了 HNT 增强纳米纤维的亲水性，有利于成纤维细胞的附着。通过 AlamarBlue 测定法进行的细胞毒性研究表明，HNT 的存在提高了纳米纤维的生物相容性。还得出结论，乙二醛可安全地用于交联壳聚糖/PVA 纳米纤维，而不会对成纤维细胞产生任何细胞毒性作用。从这项工作的结果可以看出，用乙二醛交联的 HNT 增强壳聚糖/PVA 纳米纤维是一种有前途的用于皮肤组织再生的纳米材料。
