Ersoz Unlu Ceren, Akkoca Özlem, Tatar Ilkan, Sargon Mustafa Fevzi, Zeybek Dilara, Oguztuzun Serpil
Department of Otolaryngology, Gulhane Training and Research Hospital, Ankara, Turkey.
Department of Otolaryngology, Ankara Training and Research Hospital, Ankara, Turkey.
Eur Arch Otorhinolaryngol. 2019 Jun;276(6):1713-1719. doi: 10.1007/s00405-019-05422-8. Epub 2019 Apr 12.
Laryngeal pathologies due to cigarette smoking vary among individuals, whereas some smokers remain disease free. These differences can be explained by multiple factors among individuals. In this context, an animal study was designed to determine if there is any protective effect of aerobic exercise against the detrimental effects of cigarette smoke on laryngeal tissues.
A total of 24 male Wistar albino rats were divided into three groups of eight animals each: control (no smoke exposure), smoking (smoke exposure), and exercise (smoke exposure and exercise) groups. Histopathological (light and electron microscopy) and immunohistochemical (GSTA1, CYP1A1, CYP2E1) evaluations of the vocal folds were performed at the end of experimental period.
Exercise group revealed statistically significant decrease in edema (p = 0.03) and inflammatory cell infiltration (p = 0.02) compared to smoking group. In electron microscopic evaluation; cytoplasmic vacuoles were also present in exercise group, but were smaller than smoking group. Edema and swollen mitochondria were also less prominent in exercise group. Condensed chromatin material in the periphery of nucleus was observed only in few cells in exercise group, and observed in more cells in smoking group. GSTA1 expression was higher (p = 0.047) and CYP1A1 expression was lower (p = 0.01) in exercise group than smoking group.
Our results indicate that aerobic exercise has a protective role on the larynx against the damaging effect of cigarette smoke. Smokers who exercise regularly may be at a lower risk of cigarette smoke-related laryngeal diseases, as compared with those who do not exercise.
吸烟导致的喉部病变因人而异，而一些吸烟者并未患病。个体之间的这些差异可由多种因素解释。在此背景下，设计了一项动物研究，以确定有氧运动对香烟烟雾对喉部组织的有害影响是否具有任何保护作用。
将总共24只雄性Wistar白化大鼠分为三组，每组8只动物：对照组（无烟雾暴露）、吸烟组（烟雾暴露）和运动组（烟雾暴露且运动）。在实验期结束时，对声带进行组织病理学（光学和电子显微镜）和免疫组织化学（GSTA1、CYP1A1、CYP2E1）评估。
与吸烟组相比，运动组的水肿（p = 0.03）和炎性细胞浸润（p = 0.02）在统计学上有显著降低。在电子显微镜评估中；运动组也存在细胞质空泡，但比吸烟组小。运动组的水肿和肿胀线粒体也不那么明显。运动组仅在少数细胞中观察到细胞核周边的浓缩染色质物质，而在吸烟组中观察到更多细胞中有此现象。运动组的GSTA1表达较高（p = 0.047），CYP1A1表达较低（p = 0.01），高于吸烟组。
我们的结果表明，有氧运动对喉部具有保护作用，可抵御香烟烟雾的破坏作用。与不运动的吸烟者相比，经常运动的吸烟者患与香烟烟雾相关的喉部疾病的风险可能更低。
