在中国人群中，长链非编码 RNA 基因中的单核苷酸多态性与非小细胞肺癌有关。
SNPs in LncRNA genes are associated with non-small cell lung cancer in a Chinese population.
机构信息
School of Public Health, Shanghai Jiaotong University School of Medicine, Hongqiao International Institute of Medicine, Shanghai Tongren Hospital, Shanghai, China.
Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.
出版信息
J Clin Lab Anal. 2019 May;33(4):e22858. doi: 10.1002/jcla.22858. Epub 2019 Apr 13.
BACKGROUND
It has indicated that single nuclear polymorphisms (SNPs) in the regions encoding non-coding transcripts are associated with lung cancer susceptibility. In a previous microarray study, we identified 13 differentially expressed long non-coding RNAs (lncRNAs) in non-small cell lung cancer (NSCLC) and associations of SNPs in these lncRNA genes with lung cancer were unknown. We conducted a case-control study to address this issue.
METHODS
Using the TaqMan method, we genotyped 17 SNPs located in the 13 lncRNA genes in 1294 cases with NSCLC and 1729 healthy controls. Unconditional logistic regression and Cox proportional hazards regression were used to analyze the associations of these SNPs with NSCLC risk and patient survival, respectively. These analyses were also repeated in subgroups of cases and controls stratified by gender, age group, smoking status, disease stage, and histological type.
RESULTS
We identified three SNPs associated with NSCLC risk. For SNP rs498238, CC genotype was associated with lower risk compared to TT genotype (adjusted OR = 0.33, 95%CI: 0.11-0.97, P = 0.043). For rs16901995, CT/TT genotypes were associated with lower risk compared to CC genotype in non-smokers (adjusted OR = 0.78, 95%CI: 0.62-0.98, P = 0.035). Variant genotypes in rs219741 were associated with NSCLC risk in young patients, and the adjusted OR was 1.47 (95%CI: 1.03-2.10, P = 0.033) when compared to the wild genotype. No SNPs were found to be associated with patient overall survival in the study.
CONCLUSION
The study suggests that some genetic polymorphisms in the lncRNA genes may influence the risk of NSCLC among Chinese.
背景
已有研究表明，非编码转录本区域的单核苷酸多态性（SNPs）与肺癌易感性相关。在之前的微阵列研究中，我们发现非小细胞肺癌（NSCLC）中有 13 个差异表达的长非编码 RNA（lncRNA），而这些 lncRNA 基因中的 SNPs 与肺癌的关联尚不清楚。我们进行了一项病例对照研究来解决这个问题。
方法
我们使用 TaqMan 方法对 1294 例 NSCLC 病例和 1729 例健康对照中的 13 个 lncRNA 基因中的 17 个 SNPs 进行了基因分型。使用非条件逻辑回归和 Cox 比例风险回归分别分析了这些 SNPs 与 NSCLC 风险和患者生存的相关性。这些分析还在按性别、年龄组、吸烟状况、疾病阶段和组织学类型分层的病例和对照亚组中重复进行。
结果
我们发现了三个与 NSCLC 风险相关的 SNPs。对于 SNP rs498238，CC 基因型与 TT 基因型相比，风险较低（调整后的 OR=0.33，95%CI：0.11-0.97，P=0.043）。对于 rs16901995，与 CC 基因型相比，非吸烟者的 CT/TT 基因型与较低的风险相关（调整后的 OR=0.78，95%CI：0.62-0.98，P=0.035）。rs219741 中的变异基因型与年轻患者的 NSCLC 风险相关，与野生基因型相比，调整后的 OR 为 1.47（95%CI：1.03-2.10，P=0.033）。在本研究中，没有发现 SNPs 与患者的总生存相关。
结论
该研究表明，lncRNA 基因中的一些遗传多态性可能影响中国人群 NSCLC 的发病风险。
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