Department of Hepatopancreatobiliary Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, PR China.
Department of Hepatopancreatobiliary Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, PR China.
Biomed Pharmacother. 2019 Jun;114:108862. doi: 10.1016/j.biopha.2019.108862. Epub 2019 Apr 11.
Small Nucleolar RNA Host Gene (SNHG16) is a novel cancer-related long noncoding RNA (lncRNA) and functions as an oncogene in a variety of cancers. Nonetheless, the expression patterns, biological function, and potential mechanisms in SNHG16 in pancreatic cancer (PC) remain rarely known. An increase in expression of SNHG16 in PC samples against adjacent normal tissues was shown here. Increased SNHG16 was linked intimately to the tumor-node-metastasis (TNM) stage, distant metastasis, tumor differentiation, and poor overall survival. Loss-of-function experiments revealed that SNHG16 knockdown suppressed the proliferation, formation of colonies, ability to migrate and invade in vitro, along with a lowered growth of the tumor in a mouse model. Mechanistically, SNHG16 might serve as a sponge competitive endogenous RNA (ceRNA) for miR-218-5p, thereby playing a role in regulating the expression of high mobility group box 1 (HMGB1) expression, a known direct miR-218-5p target in PC cells. These results provide novel insight into PC tumorigenesis and suggest that SNHG16 could serve as a likely therapeutic intervention in PC.
小核仁 RNA 宿主基因 (SNHG16) 是一种新型的癌症相关长非编码 RNA (lncRNA)，在多种癌症中作为癌基因发挥作用。然而，SNHG16 在胰腺癌 (PC) 中的表达模式、生物学功能和潜在机制仍知之甚少。本研究显示，PC 样本中的 SNHG16 表达增加，而相邻正常组织中的 SNHG16 表达则降低。SNHG16 的增加与肿瘤-淋巴结-转移 (TNM) 分期、远处转移、肿瘤分化和总体生存不良密切相关。功能丧失实验表明，SNHG16 敲低抑制了体外细胞的增殖、集落形成、迁移和侵袭能力，同时降低了小鼠模型中肿瘤的生长。从机制上讲，SNHG16 可能作为 miR-218-5p 的竞争性内源性 RNA (ceRNA)，从而在调节高迁移率族蛋白 B1 (HMGB1) 表达中发挥作用，HMGB1 是 PC 细胞中已知的直接 miR-218-5p 靶标。这些结果为 PC 肿瘤发生提供了新的见解，并表明 SNHG16 可能成为 PC 的一种潜在治疗干预靶点。
