Asano Y, Nakamoto Y
Clin Nephrol. 1978 Oct;10(4):123-9.
Ten patients with clinically active SLE and biopsy-proven renal involvement were studied. Antigen binding capacity (ABC) and avidity of anti-nDNA antibodies were measured, using 125I-nDNA, by the ammonium sulfate test of Minden and Farr. The glomerular localization of immune complexes was determined by morphological studies. Mean avidity in 7 patients with mesangial and subendothelial deposits was 0.387 +/- 0.022 (mean +/- SE), and in those with additional subepithelial deposits was 0.247 +/- 0.036 (P less than 0.01). When measured serially, the avidity was found to alter slowly either during treatment or spontaneously. Follow-up histology in 2 cases showed morphologic transitions; in one the transition from the initial mesangial proliferative type to the membranous form was associated with a shift of the avidity from a high level to a low one. There was no significant relation between the avidity and ABC (r = 0.235, P greater than 0.1), suggesting that avidity and the quantitative ABC of antibodies in immune complexes may not necessarily behave in parallel. Thus, the avidity measurement is useful in understanding the immunological events which underlie various clinicopathological features of SLE.
对10例临床活动期系统性红斑狼疮（SLE）且经活检证实有肾脏受累的患者进行了研究。采用125I标记的天然DNA（nDNA），通过明登和法尔的硫酸铵试验测定抗nDNA抗体的抗原结合能力（ABC）和亲和力。通过形态学研究确定免疫复合物在肾小球的定位。7例有系膜和内皮下沉积物患者的平均亲和力为0.387±0.022（均值±标准误），有额外上皮下沉积物患者的平均亲和力为0.247±0.036（P<0.01）。连续测量时，发现亲和力在治疗期间或自发情况下变化缓慢。2例患者的随访组织学显示有形态学转变；其中1例从最初的系膜增生型转变为膜型，同时亲和力从高水平转变为低水平。亲和力与ABC之间无显著相关性（r = 0.235，P>0.1），这表明亲和力与免疫复合物中抗体的定量ABC不一定平行。因此，亲和力测量有助于理解SLE各种临床病理特征背后的免疫事件。
