Banerjee P C
Arch Microbiol. 1986 Sep;145(4):408-10. doi: 10.1007/BF00470880.
Biosynthesis of alginic acid in presence of metabolic inhibitors by resting cells of mucoid Pseudomonas aeruginosa was studied. Among the inhibitors tested, arsenite exhibited very interesting results, while the others showed no remarkable effect. Firstly, arsenite stopped alginate production from all the substrates during initial hours of incubation; secondly, degradation of newly synthesized alginates to smaller molecular weight fragments took place if it was added after a few hours of incubation with the substrate; and thirdly, uncontrolled synthesis of alginate started after several hours of inhibition. Presence of arsenite was needed for the initial inhibitory phase of alginate synthesis; but once the cells were capable of synthesizing alginate after initial hours of inhibition, arsenite may be omitted from the medium.
研究了在代谢抑制剂存在的情况下，黏液型铜绿假单胞菌静止细胞中藻酸的生物合成。在所测试的抑制剂中，亚砷酸盐呈现出非常有趣的结果，而其他抑制剂则未显示出显著效果。首先，在培养初期，亚砷酸盐会阻止所有底物产生藻酸盐；其次，如果在与底物培养数小时后添加亚砷酸盐，新合成的藻酸盐会降解为分子量较小的片段；第三，在抑制数小时后，藻酸盐会开始不受控制地合成。藻酸盐合成的初始抑制阶段需要亚砷酸盐的存在；但一旦细胞在抑制初期能够合成藻酸盐，培养基中就可以省略亚砷酸盐。
