Banerjee P C, Vanags R I, Chakrabarty A M, Maitra P K
J Bacteriol. 1985 Jan;161(1):458-60. doi: 10.1128/jb.161.1.458-460.1985.
We have isolated a mutant of Pseudomonas aeruginosa deficient in fructose 1,6-bisphosphate aldolase activity. This mutant, similar to the mutants deficient in any of the Entner-Doudoroff pathway enzymes, does not allow appreciable alginate formation from glucose and gluconate, but allows alginate synthesis from mannitol and fructose. This suggests that glucose and gluconate must be converted to fructose 1,6-bisphosphate via the Entner-Doudoroff pathway enzymes and fructose 1,6-bisphosphate aldolase.
我们分离出了一株铜绿假单胞菌突变体，其缺乏1,6 - 二磷酸果糖醛缩酶活性。该突变体与缺乏Entner - Doudoroff途径中任何一种酶的突变体相似，不能利用葡萄糖和葡萄糖酸盐大量合成藻酸盐，但能利用甘露醇和果糖合成藻酸盐。这表明葡萄糖和葡萄糖酸盐必须通过Entner - Doudoroff途径的酶和1,6 - 二磷酸果糖醛缩酶转化为1,6 - 二磷酸果糖。
