Kouame Kofi, Peter Aniekan Imo, Akang Edidiong Nnamso, Adana Misturah, Moodley Roshila, Naidu Edwin Coleridge, Azu Onyemaechi Okpara
Discipline of Clinical Anatomy, School of Laboratory Medicine and Medical Sciences, University of KwaZulu-Natal , Durban , South Africa.
Department of Anatomy, College of Medicine, University of Lagos , Lagos , Nigeria.
Turk J Biol. 2018 Dec 10;42(6):498-505. doi: 10.3906/biy-1805-103. eCollection 2018.
This study investigated the toxic effects of silver on the kidneys and livers of Sprague-Dawley rats after administering multiple doses of silver nanoparticles synthesized using extracts of Cinnamomum cassia (CcAgNPs). Twenty-four Sprague-Dawley rats (250 ± 20 g) were randomly assigned to four groups (A-D) of six animals per group and treated for 8 weeks. Group A was administered 200 mg/kg of Cinnamon Cassia extract (Cc), group B 5 mg/kg of CcAgNPs, group C 10 mg/kg of CcAgNPs, and group D normal saline. Body weight was measured weekly and fasting blood glucose was measured fortnightly. At the end of the experiment, animals were euthanized and organs (livers and kidneys) were fixed in neutral buffered formalin and processed for light microscopy (H&E). Body weight differences were significantly higher (P < 0.05) in the low-dose Cc group and the kidney to body weight ratio was not significant. Renal function analysis of proteins and ketones showed a significant increase in CcAgNP-treated rats (P < 0.05). Kidney and liver histology showed distortions in hepatocytes and sinusoidal linings with infiltrations especially in the higher dose groups. Kidney histology mirrored degenerative changes in glomerular and Bowman's capsules with bfirillary mesangial interstitium. CcAgNPs impairs renal and hepatic morphology and function after a long period of administration.
本研究调查了多次给予用肉桂提取物合成的银纳米颗粒（CcAgNPs）后，银对Sprague-Dawley大鼠肾脏和肝脏的毒性作用。将24只Sprague-Dawley大鼠（250±20克）随机分为四组（A-D），每组6只动物，治疗8周。A组给予200毫克/千克肉桂提取物（Cc），B组给予5毫克/千克CcAgNPs，C组给予10毫克/千克CcAgNPs，D组给予生理盐水。每周测量体重，每两周测量空腹血糖。实验结束时，对动物实施安乐死，并将器官（肝脏和肾脏）固定在中性缓冲福尔马林中，进行光学显微镜检查（苏木精-伊红染色）。低剂量Cc组的体重差异显著更高（P<0.05），肾脏与体重之比不显著。蛋白质和酮的肾功能分析显示，CcAgNP处理的大鼠有显著增加（P<0.05）。肾脏和肝脏组织学显示，肝细胞和窦状隙内衬有扭曲，尤其是在高剂量组有浸润。肾脏组织学反映了肾小球和鲍曼囊的退行性变化以及有纤维状的系膜间质。长期给药后，CcAgNPs会损害肾脏和肝脏的形态和功能。
