Raad Maya, Glare Travis R, Brochero Helena L, Müller Caroline, Rostás Michael
Bio-Protection Research Centre, Lincoln University, Lincoln, New Zealand.
Facultad de Ciencias Agrarias, Universidad Nacional de Colombia, Bogotá, Colombia.
Front Microbiol. 2019 Mar 29;10:615. doi: 10.3389/fmicb.2019.00615. eCollection 2019.
The entomopathogenic fungus can adopt an endophytic lifestyle by colonising a wide array of plant species. -colonised plants can show enhanced resistance against insects and plant pathogens alike. However, little is known about the molecular and physiological mechanisms that govern such interactions. Here, we assessed the effects of two strains (BG11, FRh2) on the growth of and its resistance against two herbivore species and a phytopathogen. Plant responses were studied on the transcriptomic and metabolic level using microarrays and by measuring changes in defence-related phytohormones and glucosinolates (GLSs). Root inoculation with BG11 significantly increased plant growth, while FRh2 had no such effect. Both strains decreased leaf lesion area caused by the phytopathogen but did not affect population growth of the aphid or the growth of caterpillars. Microarray analyses of leaves from endophyte-inoculated provided evidence for transcriptional reprogramming of plant defence pathways, with strain-specific changes in the expression of genes related to pathogenesis, phytoalexin, jasmonic (JA), and salicylic acid (SA) signalling pathways. However, colonisation did not result in higher concentrations of JA and SA or major changes in leaf GLS profiles. We conclude that the endophyte induces plant defence responses and hypothesise that these contribute to enhanced resistance against .
昆虫病原真菌可通过定殖于多种植物物种而采取内生生活方式。被定殖的植物对昆虫和植物病原体均表现出增强的抗性。然而，对于调控此类相互作用的分子和生理机制知之甚少。在此，我们评估了两种菌株（BG11、FRh2）对[植物名称]生长及其对两种食草动物物种和一种植物病原体抗性的影响。利用微阵列并通过测量防御相关植物激素和芥子油苷（GLSs）的变化，在转录组和代谢水平上研究了植物的反应。用BG11接种根部显著促进了植物生长，而FRh2则无此效果。两种菌株均减小了由植物病原体[病原体名称]引起的叶片病斑面积，但不影响蚜虫[蚜虫名称]的种群增长或[毛虫名称]毛虫的生长。对内生菌接种的[植物名称]叶片进行微阵列分析，为植物防御途径的转录重编程提供了证据，在与发病机制、植保素、茉莉酸（JA）和水杨酸（SA）信号通路相关的基因表达上存在菌株特异性变化。然而，[真菌名称]定殖并未导致JA和SA浓度升高，也未使叶片GLS谱发生重大变化。我们得出结论，内生菌[真菌名称]诱导了植物防御反应，并推测这些反应有助于增强对[病原体名称]的抗性。
