Biotechnology Research Center, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, Southwest University, Chongqing, P. R. China.
Pest Manag Sci. 2021 Apr;77(4):2007-2018. doi: 10.1002/ps.6229. Epub 2021 Jan 12.
It has been suggested that entomopathogenic fungi can be introduced into plants as endophytes potentially leading to insect control. Here, we sought to identify specific strains of the insect pathogenic fungus, Beauveria bassiana that would form endophytic associations with tobacco (Nicotiana benthamiana) benefitting host plant growth and/or resistance against insect pests and pathogens.
Tobacco seeds were inoculated with six different B. bassiana strains and entophytic colonization, plant growth, and resistance to pathogens and insect pests were evaluated over a 50 day-period. Although all the strains could colonize seedlings, 90% seedling colonization was seen for four strains. Fungal cells could be detected in stems more readily than in leaf and root tissues. Colonization by B. bassiana boosted plant growth with an increased photosynthetic rate, chlorophyll content, and stomatal and trichome density seen in fungal treated plants. Tobacco seedlings colonized by specific B. bassiana strains displayed significantly increased tolerance/resistance against bacterial and fungal pathogens. B. bassiana-colonized seedlings also displayed higher resistance to aphids (Myzus persicae) as compared to untreated controls. Colonization by B. bassiana was shown to trigger both of the salicylic acid (SA) and jasmonate acid (JA) defense pathways, but SA pathway was upregulated much more than JA pathway for some of the tested strains.
Specific strains of B. bassiana can be introduced into host plants as endophytes, resulting in promotion of host plant growth, increased resistance to microbial pathogens, and/or increased resistance to insect pests. © 2020 Society of Chemical Industry.
有研究表明，昆虫病原真菌可以作为内生真菌引入植物，从而可能达到控制昆虫的目的。在这里，我们试图鉴定出能够与烟草（Nicotiana benthamiana）形成内生关系的特定昆虫病原真菌白僵菌（Beauveria bassiana）菌株，从而促进宿主植物的生长和/或增强对害虫和病原体的抗性。
将 6 种不同的白僵菌菌株接种到烟草种子中，在 50 天的时间内评估内生菌定殖、植物生长以及对病原体和害虫的抗性。虽然所有菌株都可以定殖幼苗，但有 4 种菌株的 90%幼苗被定殖。在茎中比在叶片和根部组织中更容易检测到真菌细胞。白僵菌的定殖促进了植物的生长，使处理过的植物的光合速率、叶绿素含量、气孔和毛状体密度增加。被特定白僵菌菌株定殖的烟草幼苗对细菌和真菌病原体的耐受性/抗性显著增强。与未处理的对照相比，白僵菌定殖的幼苗对蚜虫（Myzus persicae）的抗性也更高。白僵菌的定殖被证明可以触发水杨酸（SA）和茉莉酸（JA）防御途径，但对于一些测试的菌株，SA 途径的上调幅度远高于 JA 途径。
特定的白僵菌菌株可以作为内生菌引入宿主植物，从而促进宿主植物的生长，增强对微生物病原体的抗性，和/或增强对害虫的抗性。© 2020 英国化学学会。
