Department of Medical Biochemistry, University of Belgrade - Faculty of Pharmacy, Belgrade 11221, Serbia.
Center of Medical Biochemistry, Clinical Center of Serbia, Belgrade 11000, Serbia.
Dis Markers. 2019 Mar 7;2019:4864370. doi: 10.1155/2019/4864370. eCollection 2019.
Influence of A736V and (C282Y and H63D) polymorphisms on serum hepcidin-25 levels and iron status parameters in end-stage renal disease (ESRD) patients stratified according to gender has not been previously investigated. In addition, we aimed to evaluate the diagnostic accuracy of the parameters to separate iron-deficiency anemia (IDA) from anemia of chronic disease.
Iron status parameters and genetic analysis were performed in 126 ESRD patients and in 31 IDA patients as the control group.
ESRD patients had significantly higher ferritin and hepcidin-25 (<0.001) relative to IDA patients. Cut-off values with the best diagnostic accuracy were found for hepcidin ≥9.32 ng/mL, ferritin ≥48.2 g/L, transferrin saturation ≥16.8%, and MCV ≥81 fL. Interaction between gender and haplotypes for the hepcidin-25 and ferritin levels in ESRD patients ( = 0.005, partial eta squared = 0.09; = 0.027, partial eta squared = 0.06, respectively) was found. Serum transferrin was influenced by the combined effect of gender and A736V polymorphism in ESRD patients ( = 0.002, partial eta squared = 0.07).
Our findings could contribute to the further investigation of mechanisms involved in the pathophysiology and important gender-related involvement of the and polymorphisms on anemia in ESRD patients.
A736V 及（C282Y 和 H63D）多态性对终末期肾病（ESRD）患者血清铁调素-25 水平和铁状态参数的影响，按性别分层尚未进行研究。此外，我们旨在评估这些参数区分缺铁性贫血（IDA）和慢性病性贫血的诊断准确性。
对 126 例 ESRD 患者和 31 例 IDA 患者（对照组）进行铁状态参数和基因分析。
ESRD 患者的铁蛋白和铁调素-25 显著高于 IDA 患者（<0.001）。发现最佳诊断准确性的截断值为铁调素≥9.32ng/ml、铁蛋白≥48.2μg/L、转铁蛋白饱和度≥16.8%和 MCV≥81fl。ESRD 患者的铁调素-25 和铁蛋白水平的性别与单倍型之间存在交互作用（=0.005，偏 eta 平方=0.09；=0.027，偏 eta 平方=0.06）。血清转铁蛋白受 ESRD 患者性别和 A736V 多态性联合效应的影响（=0.002，偏 eta 平方=0.07）。
我们的研究结果可能有助于进一步研究涉及 ESRD 患者病理生理学的机制以及 与 多态性在贫血方面的重要性别相关作用。
