一种用于直接检测嘌呤抗代谢物和DNA降解的新型电化学传感器。

A New Electrochemical Sensor for Direct Detection of Purine Antimetabolites and DNA Degradation.

作者信息

Shpigun Liliya K, Andryukhina Elena Yu

机构信息

Institute of General & Inorganic Chemistry of Russian Academy of Sciences, 119991 Moscow, Russia.

出版信息

J Anal Methods Chem. 2019 Mar 7;2019:1572526. doi: 10.1155/2019/1572526. eCollection 2019.

The development of a reliable electrochemical sensor using a hybrid nanocomposite consisting of ionic liquid (1-butyl-3-methylimidazolium hexafluorophosphate) functionalized graphene oxide (GrO-IL) and gold nanoparticles (AuNPs) stabilized by chitosan (Chit) was described. The new sensor, labelled as GrO-IL-AuNPs-Chit/CSE, exhibited an improved electrocatalytic response to cancer drugs such as purine antimetabolites (6-thioguanine, 6-mercaptopurine, and azathioprine) in a wide concentration range with a low detection limit (20-40 nmol·L, S/N = 3), and satisfactory recoveries (97.1-103.0%). The sensor has been also successfully used for cyclic voltammetric study of a salmon sperm double-stranded DNA degradation and DNA-6-mercaptopurine interaction in aqueous solutions (pH 7.4).

描述了一种可靠的电化学传感器的研制，该传感器使用了一种混合纳米复合材料，其由离子液体（1-丁基-3-甲基咪唑六氟磷酸盐）功能化的氧化石墨烯（GrO-IL）和由壳聚糖（Chit）稳定的金纳米颗粒（AuNPs）组成。这种标记为GrO-IL-AuNPs-Chit/CSE的新型传感器，在较宽的浓度范围内对嘌呤抗代谢物（6-硫鸟嘌呤、6-巯基嘌呤和硫唑嘌呤）等抗癌药物表现出改善的电催化响应，检测限低（20 - 40 nmol·L，S/N = 3），回收率令人满意（97.1 - 103.0%）。该传感器还成功用于鲑鱼精子双链DNA降解以及水溶液（pH 7.4）中DNA - 6-巯基嘌呤相互作用的循环伏安研究。