具有增强乙醇耐受性的ZM4突变体的表型和基因组分析。
Phenotypic and genomic analysis of ZM4 mutants with enhanced ethanol tolerance.
作者信息
Carreón-Rodríguez Ofelia E, Gutiérrez-Ríos Rosa María, Acosta José L, Martinez Alfredo, Cevallos Miguel A
机构信息
Programa de Genómica Evolutiva, Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Apartado Postal 565-A, Cuernavaca, Morelos, Mexico.
Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, Cuernavaca, Morelos, 62210, Mexico.
出版信息
Biotechnol Rep (Amst). 2019 Mar 21;23:e00328. doi: 10.1016/j.btre.2019.e00328. eCollection 2019 Sep.
ZM4 is an ethanol-producing microbe that is constitutively tolerant to this solvent. For a better understanding of the ethanol tolerance phenomenon we obtained and characterized two ZM4 mutants (ER79ap and ER79ag) with higher ethanol tolerance than the wild-type. Mutants were evaluated in different ethanol concentrations and this analysis showed that mutant ER79ap was more tolerant and had a better performance in terms of cell viability, than the wild-type strain and ER79ag mutant. Genotyping of the mutant strains showed that both carry non-synonymous mutations in and genes. A third non-synonymous mutation was found only in strain ER79ap, in the gene. Considering that ER79ap has the best tolerance to added ethanol, the mutant alleles of this strain were evaluated in ZM4 and here we show that while all of them contribute to ethanol tolerance, mutation within T/A gene seems to be the most important.
ZM4是一种产乙醇的微生物，它对这种溶剂具有组成型耐受性。为了更好地理解乙醇耐受现象，我们获得并鉴定了两个乙醇耐受性高于野生型的ZM4突变体（ER79ap和ER79ag）。在不同乙醇浓度下对突变体进行了评估，该分析表明，与野生型菌株和ER79ag突变体相比，突变体ER79ap耐受性更强，在细胞活力方面表现更好。突变菌株的基因分型表明，两者在 和 基因中都携带非同义突变。仅在ER79ap菌株的 基因中发现了第三个非同义突变。鉴于ER79ap对添加的乙醇具有最佳耐受性，对该菌株的突变等位基因在ZM4中进行了评估，我们在此表明，虽然所有这些等位基因都有助于乙醇耐受性，但T/A基因内的突变似乎是最重要的。
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