MRC Cancer Unit, University of Cambridge, Hutchison/MRC Research Centre, Box 197, Cambridge Biomedical Campus, Cambridge, CB2 0XZ, UK.
Endomagnetics Ltd., The Jeffreys Building, St John's Innovation Park, Cowley Road, Cambridge, CB4 0WS, UK.
Small. 2019 May;15(20):e1900224. doi: 10.1002/smll.201900224. Epub 2019 Apr 15.
Interstitially administered iron oxide particles are currently used for interoperative localization of sentinel lymph nodes (LNs) in cancer staging. Several studies have described concerns regarding the cellular accumulation of iron oxide nanoparticles relating them to phenotype and function deregulation of macrophages, impairing their ability to mount an appropriate immune response once an insult is present. This study aims to address what phenotypic and functional changes occur during lymphatic transit and accumulation of these particles. Data show that 60 nm carboxydextran-coated iron nanoparticles use a noncellular mechanism to reach the draining LNs and that their accumulation in macrophages induces transient phenotypic and functional changes. Nevertheless, macrophages recover their baseline levels of response within 7 days, and are still able to mount an appropriate response to bacterially induced inflammation.
目前，用于癌症分期手术中前哨淋巴结（LNs）定位的是间质内给药的氧化铁颗粒。有几项研究描述了人们对氧化铁纳米颗粒细胞内积累的担忧，这些颗粒与巨噬细胞表型和功能的失调有关，一旦受到刺激，就会损害它们产生适当免疫反应的能力。本研究旨在探讨这些颗粒在淋巴转运和积累过程中发生了哪些表型和功能变化。数据表明，60nm 羧基葡聚糖包覆的铁纳米颗粒使用非细胞机制到达引流 LNs，并且它们在巨噬细胞中的积累诱导了短暂的表型和功能变化。然而，巨噬细胞在 7 天内恢复到基线反应水平，并且仍然能够对细菌诱导的炎症做出适当的反应。
