Ho C K, Babiuk L A
Immunology. 1978 Nov;35(5):733-40.
Various techniques were used to separate canine peripheral blood leucocytes into populations enriched in lymphocytes, polymorphonuclear leucocytes, phagocytic mononuclear cells (monocytes) and macrophages. Surface markers on each cell population were determined by rosette formation. Fc receptors for IgG and complement receptors (C3b and C3d) were present on PMN, monocytes, macrophages as well as on a sub-population of lymphocytes. Purification of the lymphocytes into T-and B-cell-enriched populations revealed that these receptors were present only on the B lymphocytes and not on the T lymphocytes. In addition, a third lymphocyte population, which did not possess surface immunoglobulin, and Fc receptor but not the complement receptor. None of the cell populations exhibited C4 complement receptors or Fc receptors for IgM. When different cell populations were tested for their ability to form rosettes directly with human type 'O' red blood cells it was found that most populations could rosette, suggesting that this technique could not be used as a specific marker for canine T lymphocytes.
采用了多种技术将犬外周血白细胞分离成富含淋巴细胞、多形核白细胞、吞噬单核细胞（单核细胞）和巨噬细胞的群体。通过玫瑰花结形成来确定每个细胞群体的表面标志物。PMN、单核细胞、巨噬细胞以及淋巴细胞亚群上存在IgG的Fc受体和补体受体（C3b和C3d）。将淋巴细胞纯化到富含T细胞和B细胞的群体中发现，这些受体仅存在于B淋巴细胞上，而不存在于T淋巴细胞上。此外，还有第三种淋巴细胞群体，它不具有表面免疫球蛋白和Fc受体，但具有补体受体。没有一个细胞群体表现出C4补体受体或IgM的Fc受体。当测试不同细胞群体直接与人“O”型红细胞形成玫瑰花结的能力时，发现大多数群体都能形成玫瑰花结，这表明该技术不能用作犬T淋巴细胞的特异性标志物。
