Ho C K, Babiuk L A
Immunology. 1979 May;37(1):231-9.
Canine peripheral blood lymphocytes, polymorphonuclear leucocytes (PMN) and monocytes (macrophages) were obtained by various cell separation techniques and were tested for their cytotoxic capacity against antibody-sensitized canine distemper virus (CDV) infected Vero cells by an in vitro chromium release assay. Canine lymphocytes were found to destroy CDV infected target cells effectively, while neither PMN nor monocytes (macrophages) could do so. The active lymphocyte was characterized by various rosetting techniques to be a non-T and a non-B lymphocyte. These cells bear no surface immunoglobulin (SIg-) but possessed both Fc receptors (Fc+) and complement receptors (EAC+) suggesting that these cells are neither classical T nor B cells. The possible roles of this K cell in the resistance against canine distemper are discussed.
通过多种细胞分离技术获取犬外周血淋巴细胞、多形核白细胞(PMN)和单核细胞(巨噬细胞),并采用体外铬释放试验检测它们对抗体致敏的犬瘟热病毒(CDV)感染的Vero细胞的细胞毒性能力。发现犬淋巴细胞能有效破坏感染CDV的靶细胞,而PMN和单核细胞(巨噬细胞)均不能。通过多种玫瑰花结技术鉴定,活性淋巴细胞为非T非B淋巴细胞。这些细胞不带有表面免疫球蛋白(SIg-),但同时具有Fc受体(Fc+)和补体受体(EAC+),提示这些细胞既不是经典的T细胞也不是B细胞。讨论了这种K细胞在抵抗犬瘟热中的可能作用。