Lin Senjie, Yu Liying, Zhang Huan
Department of Marine Sciences, University of Connecticut, Groton, CT 06340, USA.
State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361102, Fujian, China.
Microorganisms. 2019 Apr 2;7(4):96. doi: 10.3390/microorganisms7040096.
Coral reef-associated Symbiodiniaceae live in tropical and oligotrophic environments and are prone to heat and nutrient stress. How their metabolic pathways respond to pulses of warming and phosphorus (P) depletion is underexplored. Here, we conducted RNA-seq analysis to investigate transcriptomic responses to thermal stress, phosphate deprivation, and organic phosphorus (OP) replacement in . Using dual-algorithm (edgeR and NOIseq) to remedy the problem of no replicates, we conservatively found 357 differentially expressed genes (DEGs) under heat stress, potentially regulating cell wall modulation and the transport of iron, oxygen, and major nutrients. About 396 DEGs were detected under P deprivation and 671 under OP utilization, both mostly up-regulated and potentially involved in photosystem and defensome, despite different KEGG pathway enrichments. Additionally, we identified 221 genes that showed relatively stable expression levels across all conditions (likely core genes), mostly catalytic and binding proteins. This study reveals a wide range of, and in many cases previously unrecognized, molecular mechanisms in to cope with heat stress and phosphorus-deficiency stress. Their quantitative expression dynamics, however, requires further verification with triplicated experiments, and the data reported here only provide clues for generating testable hypotheses about molecular mechanisms underpinning responses and adaptation in to temperature and nutrient stresses.
与珊瑚礁相关的共生甲藻生活在热带和贫营养环境中，容易受到热和营养胁迫。它们的代谢途径如何应对变暖和磷（P）耗竭的脉冲尚未得到充分研究。在这里，我们进行了RNA测序分析，以研究[具体对象]对热胁迫、磷酸盐剥夺和有机磷（OP）替代的转录组反应。使用双算法（edgeR和NOIseq）来解决无重复样本的问题，我们保守地发现热胁迫下有357个差异表达基因（DEG），可能调节细胞壁调节以及铁、氧和主要营养物质的运输。在缺磷条件下检测到约396个DEG，在利用OP条件下检测到671个DEG，尽管KEGG途径富集不同，但两者大多上调，可能参与光系统和防御组。此外，我们鉴定出221个在所有条件下表达水平相对稳定的基因（可能是核心基因），大多是催化蛋白和结合蛋白。这项研究揭示了[具体对象]应对热胁迫和缺磷胁迫的广泛且在许多情况下以前未被认识的分子机制。然而，它们的定量表达动态需要通过重复实验进一步验证，这里报告的数据仅为生成关于[具体对象]对温度和营养胁迫的反应及适应基础分子机制的可测试假设提供线索。
