Howell D N, Salter R D, Koppelman B D, Cresswell P
Hum Immunol. 1986 Dec;17(4):443-55. doi: 10.1016/0198-8859(86)90303-4.
In previous communications, we have described the expression of class I and class II histocompatibility antigens by hybrids of human B and T lymphoblastoid cell lines (B- and T-LCL). In all cases, such hybrids were found to resemble their B-LCL parents, expressing high levels of class I and class II antigens encoded by both parent cell lines. In the current study, we have conducted a more extensive analysis of B-LCLxT-LCL hybrids with a panel of monoclonal antibodies recognizing a variety of B and T lymphocyte differentiation markers. Rather than exhibiting a B-LCL-dominant phenotype, most hybrids were found to express a majority of both T and B lymphocyte antigens expressed by their parent cell lines. Several hybrids of pairs of dissimilar T-LCL were also produced and analyzed. Again, a majority of parental antigens was expressed on the hybrids. However, eight of eight hybrids of the T-LCL CEM and HSB failed to express HNK-1, an antigen strongly expressed by HSB; and two hybrids of the T-LCL CEM and SKW3 expressed CD3, an antigen expressed by neither parent cell line.
在之前的通讯中，我们描述了人B淋巴母细胞系和T淋巴母细胞系（B-LCL和T-LCL）杂交体中I类和II类组织相容性抗原的表达情况。在所有情况下，发现此类杂交体与其B-LCL亲本相似，表达由两个亲本细胞系编码的高水平I类和II类抗原。在当前研究中，我们使用一组识别多种B和T淋巴细胞分化标志物的单克隆抗体，对B-LCL×T-LCL杂交体进行了更广泛的分析。大多数杂交体并未表现出B-LCL主导的表型，而是被发现表达其亲本细胞系所表达的大多数T和B淋巴细胞抗原。还产生并分析了几对不同T-LCL的杂交体。同样，大多数亲本抗原在杂交体上表达。然而，T-LCL CEM和HSB的八对杂交体中的八个未能表达HSB强烈表达的抗原HNK-1；T-LCL CEM和SKW3的两个杂交体表达了CD3，这是一种两个亲本细胞系均未表达的抗原。
