用于生物医学应用的生物相容性复合材料的样品制备技术。
A Sample Preparation Technique Using Biocompatible Composites for Biomedical Applications.
机构信息
Department of Convergence Medicine, Asan Medical Institute of Convergence Science and Technology (AMIST), University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul 05505, Korea.
Biomedical Engineering Research Center, Asan Institute of Life Sciences, Asan Medical Center, 88 Olympicro-43gil, Songpa-gu, Seoul 05505, Korea.
出版信息
Molecules. 2019 Apr 3;24(7):1321. doi: 10.3390/molecules24071321.
Infectious diseases, especially pathogenic infections, are a growing threat to public health worldwide. Since pathogenic bacteria usually exist in complex matrices at very low concentrations, the development of technology for rapid, convenient, and biocompatible sample enrichment is essential for sensitive diagnostics. In this study, a cucurbit[6]uril (CB) supermolecular decorated amine-functionalized diatom (DA) composite was fabricated to support efficient sample enrichment and in situ nucleic acid preparation from enriched pathogens and cells. CB was introduced to enhance the rate and effectiveness of pathogen absorption using the CB-DA composite. This novel CB-DA composite achieved a capture efficiency of approximately 90% at an concentration of 10⁶ CFU/mL within 3 min. Real-time PCR analyses of DNA samples recovered using the CB-DA enrichment system showed a four-fold increase in the early amplification signal strength, and this effective method for capturing nucleic acid might be useful for preparing samples for diagnostic systems.
传染病，尤其是病原性感染，是全球公共卫生日益严重的威胁。由于病原菌通常以非常低的浓度存在于复杂的基质中，因此开发用于快速、方便和生物相容的样品富集的技术对于敏感诊断至关重要。在这项研究中，制备了一种葫芦[6]脲（CB）超分子修饰的胺功能化硅藻（DA）复合材料，以支持从富集的病原体和细胞中进行高效的样品富集和原位核酸制备。引入 CB 以提高使用 CB-DA 复合材料吸收病原体的速度和效率。在 浓度为 10⁶ CFU/mL 时，新型 CB-DA 复合材料在 3 分钟内达到了约 90%的捕获效率。使用 CB-DA 富集系统回收的 DNA 样品的实时 PCR 分析显示，早期扩增信号强度增加了四倍，这种有效的核酸捕获方法可能有助于为诊断系统制备样品。
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