State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A & F University, Yangling 712100, China.
Molecules. 2019 Apr 4;24(7):1329. doi: 10.3390/molecules24071329.
Alkylresorcinols (ARs) are beneficial for human health and can be used as biomarkers for whole-grain cereal intake. In previous studies, ARs content in whole-grain flour was determined by milling the seeds into powder, thus destroying their structure. In this paper, we adopted a non-destructive extraction approach. Chloroform and ethyl acetate extraction methods were carried out to extract lipids from the seed surface and whole-grain flour of 36 wheat varieties in China, respectively. GC-MS analysis identified chemical compounds in which ARs were the major compounds for all the samples. The average total content of ARs (624.223 µg/g) in whole grains was much higher than that on seed surfaces (4.934 µg/g), with a positive correlation (r = 0.863, < 0.001) between these two parameters. The results suggested that the total ARs content on the seed surface can be used to predict their content in whole-grain flour. Without affecting the seed germination rate and damaging seed structure, we demonstrate that a non-destructive extraction approach is an appropriate and useful method, facilitating the development of rapid screening methods suitable for use in wheat breeding.
烷基间苯二酚（ARs）有益于人类健康，可作为全谷物摄入的生物标志物。在以前的研究中，通过将种子磨成粉末来确定全谷物面粉中的 ARs 含量，从而破坏了它们的结构。在本文中，我们采用了一种非破坏性的提取方法。分别采用氯仿和乙酸乙酯提取法从中国 36 种小麦品种的种子表面和全谷物面粉中提取脂质。GC-MS 分析鉴定了 ARs 是所有样品的主要化合物。全谷物中 ARs 的平均总含量（624.223 µg/g）远高于种子表面的含量（4.934 µg/g），这两个参数之间呈正相关（r = 0.863，<0.001）。结果表明，种子表面的总 ARs 含量可用于预测其在全谷物面粉中的含量。在不影响种子发芽率和破坏种子结构的情况下，我们证明了非破坏性提取方法是一种合适且有用的方法，有利于开发适合小麦育种的快速筛选方法。
