Institute of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Changsha 410013, China.
College of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, China.
Molecules. 2019 Apr 4;24(7):1331. doi: 10.3390/molecules24071331.
As an edible sclerotia-forming fungus, is widely used as a food supplement and as a tonic in China. High-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (HPLC-QTOF-MS/MS) was applied to identify triterpene acids in fermented mycelia of , as well as the epidermis and inner part of natural sclerotia. A total of 19 triterpene acids were identified in fermented mycelia, whereas 31 were identified in the epidermis and 24 in the inner part. Nine triterpene acids were quantitatively determined, and the concentrations of two valuable triterpenes, dehydropachymic acid and pachymic acid, reached 1.07 mg/g and 0.61 mg/g in the fermented mycelia part, respectively, and were both significantly higher than the concentration in the two natural parts. The fermented mycelia could be a good choice for producing some target triterpene compounds and functional foods through fermentation thanks to the high concentration of some triterpene acids.
作为一种可食用的结皮真菌，灵芝在中国被广泛用作食品补充剂和补品。高效液相色谱-四极杆/飞行时间质谱联用（HPLC-QTOF-MS/MS）被用于鉴定灵芝发酵菌丝体、表皮和天然结皮内部的三萜酸。在发酵菌丝体中鉴定出 19 种三萜酸，在表皮中鉴定出 31 种，在内部鉴定出 24 种。定量测定了 9 种三萜酸，两种有价值的三萜酸，去氢灵芝酸和灵芝酸，在发酵菌丝体部分的浓度分别达到 1.07 毫克/克和 0.61 毫克/克，均明显高于两种天然部分的浓度。由于一些三萜酸的高浓度，发酵菌丝体可以通过发酵成为生产某些目标三萜化合物和功能性食品的良好选择。
