Evaluation of the real-time fluorescence loop-mediated isothermal amplification assay for the detection of .

is a major pathogenic bacterium causing perinatal infections in humans. In the present study, a novel real-time fluorescence loop-mediated isothermal amplification technology was successfully developed and evaluated for the detection of in a single reaction. Six specific primers were designed to amplify the corresponding six regions of fbs B gene of , using Bst DNA polymerase with DNA strand displacement activity at a constant temperature for 60 min. The presence of was indicated by the fluorescence in real-time. Amplification of the targeted gene fragment was optimized with the primer 1 in the current setup. Positive result was only obtained for Sa by Real-LAMP among 10 tested relevant bacterial strains, with the detection sensitivity of 300 pg/µl. Real-LAMP was demonstrated to be a simple and rapid detection tool for with high specificity and stability, which ensures its wide application and broad prospective utilization in clinical practice for the rapid detection of .