Das Jhumki, Bhatia Prateek, Singh Aditya
1Pediatric Allergy Immunology Unit, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
2Pediatric Hematology-Oncology Unit, Advanced Paediatric Centre, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012 India.
Indian J Hematol Blood Transfus. 2019 Apr;35(2):208-214. doi: 10.1007/s12288-018-1008-z. Epub 2018 Sep 17.
Gene editing research has seen rapid growth over the past decade or so, however with the discovery of - gene editing tool in recent years, the same has witnessed a global interest with many scientists and research groups worldwide carrying out cutting edge experiments to target various diseases and cancers and develop a cure. This has been made possible partially due to the ease of use and flexibility of the - system as compared to other conventional gene editing tools. Hence, - has found its way into most basic molecular laboratories and within reach of most low-middle income research groups. Despite these favourable advantages, there exists a cost barrier and lack of proper knowledge and awareness on the correct work flow desired, especially in molecular laboratories looking forward to develop and experiment with high end research. This mini review attempts to iron out these factors and project an algorithmic approach to tide over and establish a workable in vitro gene editing experiment in a resource constraint haematology oncology laboratory setting. However, the basic principle and steps outlined in this review can also be translated for research in any other medical specialty laboratory setting.
在过去十年左右的时间里，基因编辑研究发展迅速。然而，近年来随着一种基因编辑工具的发现，全球对此产生了浓厚兴趣，世界各地的许多科学家和研究团队都在开展前沿实验，以针对各种疾病和癌症并研发治疗方法。与其他传统基因编辑工具相比，这在一定程度上得益于该系统使用简便且具有灵活性。因此，它已进入大多数基础分子实验室，大多数中低收入研究团队也能够使用。尽管有这些有利优势，但仍存在成本障碍，且对于期望开展高端研究并进行实验的分子实验室而言，缺乏对所需正确工作流程的适当了解和认识。本综述试图梳理这些因素，并提出一种算法方法，以克服困难，在资源有限的血液肿瘤学实验室环境中建立可行的体外基因编辑实验。然而，本综述中概述的基本原则和步骤也可转化用于任何其他医学专业实验室环境的研究。
