Piggot P J, Chak K F, Bugaichuk U D
J Gen Microbiol. 1986 Jul;132(7):1875-81. doi: 10.1099/00221287-132-7-1875.
The Bacillus subtilis spoVE locus was isolated from a lambda clone bank and a 4.7 kbp EcoRV fragment subcloned into the shuttle vector pHV33. The resulting plasmid complemented chromosomal spoVE mutations. Its structure was stable in recE4 strains, but plasmid and chromosomal rearrangements occurred in rec+ strains. New spoVE mutations were obtained by mutagenesis of the plasmid; all the mutations tested mapped within three adjacent HindIII fragments of total length 1140 bp.
枯草芽孢杆菌spoVE基因座是从一个λ克隆文库中分离出来的，并将一个4.7kbp的EcoRV片段亚克隆到穿梭载体pHV33中。所得质粒可互补染色体上的spoVE突变。其结构在recE4菌株中是稳定的，但在rec⁺菌株中会发生质粒和染色体重排。通过对该质粒进行诱变获得了新的spoVE突变；所有测试的突变都定位在总长度为1140bp的三个相邻HindIII片段内。
