Beall B, Lowe M, Lutkenhaus J
Department of Microbiology, University of Kansas Medical Center, Kansas City 66103.
J Bacteriol. 1988 Oct;170(10):4855-64. doi: 10.1128/jb.170.10.4855-4864.1988.
The Bacillus subtilis homolog of the Escherichia coli ftsZ gene was isolated by screening a B. subtilis genomic library with anti-E. coli FtsZ antiserum. DNA sequence analysis of a 4-kilobase region revealed three open reading frames. One of these coded for a protein that was about 50% homologous to the E. coli FtsZ protein. The open reading frame just upstream of ftsZ coded for a protein that was 34% homologous to the E. coli FtsA protein. The open reading frames flanking these two B. subtilis genes showed no relationship to those found in E. coli. Expression of the B. subtilis ftsZ and ftsA genes in E. coli was lethal, since neither of these genes could be cloned on plasmid vectors unless promoter sequences were first removed. Cloning the B. subtilis ftsZ gene under the control of the lac promoter resulted in an IPTGs phenotype that could be suppressed by overproduction of E. coli FtsZ. These genes mapped at 135 degrees on the B. subtilis genetic map near previously identified cell division mutations.
通过用抗大肠杆菌FtsZ抗血清筛选枯草芽孢杆菌基因组文库，分离出了大肠杆菌ftsZ基因的枯草芽孢杆菌同源物。对一个4千碱基区域的DNA序列分析揭示了三个开放阅读框。其中一个编码的蛋白质与大肠杆菌FtsZ蛋白约有50%的同源性。ftsZ上游的开放阅读框编码的蛋白质与大肠杆菌FtsA蛋白有34%的同源性。这两个枯草芽孢杆菌基因两侧的开放阅读框与在大肠杆菌中发现的开放阅读框没有关系。枯草芽孢杆菌ftsZ和ftsA基因在大肠杆菌中的表达是致死性的，因为除非首先去除启动子序列，否则这两个基因都不能克隆到质粒载体上。在lac启动子的控制下克隆枯草芽孢杆菌ftsZ基因导致了一种IPTG表型，这种表型可以通过过量表达大肠杆菌FtsZ来抑制。这些基因在枯草芽孢杆菌遗传图谱上位于135度，靠近先前鉴定的细胞分裂突变。
