Liu H M, Chak K F, Piggot P J
J Gen Microbiol. 1982 Nov;128(11):2805-12. doi: 10.1099/00221287-128-11-2805.
Plasmid pHM2 consists of a 3.3 kb insert of Bacillus subtilis DNA in the chimeric plasmid pHV33, and can replicate in Escherichia coli and B. subtilis. In B. subtilis, pHM2 complements the defects resulting from mutations spo-42, spo-50, spo-69 and sas-1 in the spoIIA locus. This complementation can occur in recE4 strains where recombination of the plasmid with the chromosome is prevented, and the chromosome retains the mutant allele. Thus the plasmid carries a functional part of the spoIIA locus; it does not contain the complete locus as it cannot complement several other spoIIA mutations. It is likely that the locus is complex, containing at least two genes.
质粒pHM2由嵌合质粒pHV33中一段3.3 kb的枯草芽孢杆菌DNA插入片段组成，可在大肠杆菌和枯草芽孢杆菌中复制。在枯草芽孢杆菌中，pHM2可弥补spoIIA位点上spo - 42、spo - 50、spo - 69和sas - 1突变所导致的缺陷。这种互补作用可在recE4菌株中发生，在该菌株中质粒与染色体的重组被阻止，而染色体保留突变等位基因。因此，该质粒携带spoIIA位点的一个功能部分；它不包含完整的位点，因为它不能弥补其他几个spoIIA突变。该位点可能很复杂，至少包含两个基因。
