Kim Yong Jin, Kim Yoon Young, Song Da Young, Lee Sang Hoon, Park Chan Woo, Kim Hoon, Ku Seung-Yup
1Department of Obstetrics and Gynecology, Korea University Medical Center, 73 Inchon-ro, Seongbuk-gu, Seoul, 02841 Republic of Korea.
2Department of Obstetrics and Gynecology, Seoul National University Hospital, 101 Daehak-ro, Jongno-gu, Seoul, 03080 Republic of Korea.
Tissue Eng Regen Med. 2018 Aug 28;16(2):131-139. doi: 10.1007/s13770-018-0156-4. eCollection 2019 Apr.
Provision of optimal endometrial stromal cells is essential in uterine tissue engineering. Culture of these cells is significantly influenced by gonadotropin hormones. This investigation attempted to define the proliferation profiles of murine uterine endometrial stromal cells during culture with recombinant follicle stimulating hormone (rFSH), urinary follicle stimulating hormone (uFSH), and human chorionic gonadotropin (hCG).
Murine uterine endometrial stromal cells were collected from 8-week-old mice and cultured up to 72 h, with rFSH, uFSH, or hCG. Cell cycles were analyzed by BrdU assay, and cyclin D1 expression was evaluated according to dose and duration of gonadotropin treatment.
BrdU assay showed a further inhibitory effect on murine uterine endometrial stromal cell proliferation when cultured with rFSH compared to uFSH, and a similar inhibitory proliferation profile when cultured with hCG at a specific range of concentrations. The expression of cyclin D1 of murine uterine endometrial stromal cells was down-regulated when cultured with rFSH, uFSH, or hCG, compared to control.
FSH may inhibit the proliferation of murine uterine endometrial stromal cells during culture. rFSH may have more significant inhibitory effects on the proliferation of endometrial stromal cells than uFSH. Establishing an optimal endocrine milieu is necessary using more advanced combination of female hormones for culture of this type of cells.
提供最佳的子宫内膜基质细胞在子宫组织工程中至关重要。这些细胞的培养受到促性腺激素的显著影响。本研究试图确定重组促卵泡激素（rFSH）、尿促卵泡素（uFSH）和人绒毛膜促性腺激素（hCG）培养过程中小鼠子宫子宫内膜基质细胞的增殖情况。
从小鼠子宫中收集8周龄小鼠的子宫内膜基质细胞，并分别用rFSH、uFSH或hCG培养72小时。通过BrdU检测分析细胞周期，并根据促性腺激素治疗的剂量和持续时间评估细胞周期蛋白D1的表达。
BrdU检测显示，与uFSH相比，用rFSH培养时对小鼠子宫子宫内膜基质细胞增殖有进一步的抑制作用，在特定浓度范围内用hCG培养时增殖抑制情况相似。与对照组相比，用rFSH、uFSH或hCG培养时，小鼠子宫子宫内膜基质细胞的细胞周期蛋白D1表达下调。
促卵泡激素（FSH）在培养过程中可能抑制小鼠子宫子宫内膜基质细胞的增殖。重组促卵泡激素（rFSH）对子宫内膜基质细胞增殖的抑制作用可能比尿促卵泡素（uFSH）更显著。使用更先进的女性激素组合建立最佳内分泌环境对于培养这类细胞是必要的。
