Ke Yinggen, Kang Yuanrong, Wu Mengxiao, Liu Hongbo, Hui Shugang, Zhang Qinglu, Li Xianghua, Xiao Jinghua, Wang Shiping
National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China.
Rice (N Y). 2019 Apr 15;12(1):25. doi: 10.1186/s12284-019-0283-0.
The function of Arabidopsis enhanced disease susceptibility 1 (AtEDS1) and its sequence homologs in other dicots have been extensively studied. However, it is unknown whether rice EDS1 homolog (OsEDS1) plays a role in regulating the rice-pathogen interaction.
In this study, a OsEDS1-knouckout mutant (oseds1) was characterized and shown to have increased susceptibility to Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc), suggesting the positive role of OsEDS1 in regulating rice disease resistance. However, the following evidence suggests that OsEDS1 shares some differences with AtEDS1 in its way to regulate the host-pathogen interactions. Firstly, OsEDS1 modulates the rice-bacteria interactions involving in jasmonic acid (JA) signaling pathway, while AtEDS1 regulates Arabidopsis disease resistance against biotrophic pathogens depending on salicylic acid (SA) signaling pathway. Secondly, introducing AtEDS1 could reduce oseds1 mutant susceptibility to Xoo rather than to Xoc. Thirdly, exogenous application of JA and SA cannot complement the susceptible phenotype of the oseds1 mutant, while exogenous application of SA is capable of complementing the susceptible phenotype of the ateds1 mutant. Finally, OsEDS1 is not required for R gene mediated resistance, while AtEDS1 is required for disease resistance mediated by TIR-NB-LRR class of R proteins.
OsEDS1 is a positive regulator in rice-pathogen interactions, and shares both similarities and differences with AtEDS1 in its way to regulate plant-pathogen interactions.
拟南芥增强型疾病易感性1（AtEDS1）及其在其他双子叶植物中的序列同源物的功能已得到广泛研究。然而，水稻EDS1同源物（OsEDS1）是否在调节水稻与病原体的相互作用中发挥作用尚不清楚。
在本研究中，对一个OsEDS1敲除突变体（oseds1）进行了表征，结果表明其对水稻白叶枯病菌（Xanthomonas oryzae pv. oryzae，Xoo）和水稻条斑病菌（Xanthomonas oryzae pv. oryzicola，Xoc）的易感性增加，这表明OsEDS1在调节水稻抗病性中起积极作用。然而，以下证据表明OsEDS1在调节宿主与病原体相互作用的方式上与AtEDS1存在一些差异。首先，OsEDS1调节涉及茉莉酸（JA）信号通路的水稻与细菌的相互作用，而AtEDS1则依赖水杨酸（SA）信号通路调节拟南芥对活体营养型病原体的抗病性。其次，导入AtEDS1可以降低oseds1突变体对Xoo的易感性，而不是对Xoc的易感性。第三，外源施用JA和SA不能弥补oseds1突变体的感病表型，而外源施用SA能够弥补ateds1突变体的感病表型。最后，R基因介导的抗性不需要OsEDS1，而TIR-NB-LRR类R蛋白介导抗病性则需要AtEDS1。
OsEDS1是水稻与病原体相互作用中的一个正向调节因子，在调节植物与病原体相互作用的方式上与AtEDS1既有相似之处，也有不同之处。
