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海岛棉增强疾病易感性1基因编码一种类似脂肪酶的蛋白质，通过调节水杨酸（SA）水平和过氧化氢（H₂O₂）积累在对（病原体）的应答中起关键作用。 （注：原文中“by Regulating the SA Level and HO Accumulation.”里的“HO”推测应该是“H₂O₂”，不然逻辑不完整，已在译文中补充完整使其符合语境）

Island Cotton Enhanced Disease Susceptibility 1 Gene Encoding a Lipase-Like Protein Plays a Crucial Role in Response to by Regulating the SA Level and HO Accumulation.

作者信息

Yan Zhang, Xingfen Wang, Wei Rong, Jun Yang, Zhiying Ma

机构信息

Department of Agronomy, North China Key Laboratory for Germplasm Resources of Education Ministry, Hebei Agricultural University Baoding, China.

出版信息

Front Plant Sci. 2016 Dec 15;7:1830. doi: 10.3389/fpls.2016.01830. eCollection 2016.

DOI:10.3389/fpls.2016.01830
PMID:28018374
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5156716/
Abstract

Cotton is one of the most economically important crops, but most cultivated varieties lack adequate innate immunity or resistance to wilt. This results in serious losses to both yield and fiber quality. To identify the genetic resources for innate immunity and understand the pathways for pathogen defenses in this crop, here we focus on orthologs of the central defense regulator 1 (). The full-length cDNA of was obtained by screening the full-length cDNA library of combining with RACE strategy. Its open reading frame is 1848 bp long, encoding 615 amino acid residues. Sequence analysis showed that GbEDS1 contains a conserved N-terminal lipase domain and an EDS1-specific KNEDT motif. Expression profiling indicated that the gene is induced by as well as salicylic acid (SA) treatment. Subcellular localization assays revealed that GbEDS1 is located in the cell cytoplasm and nucleus. Overexpression of in dramatically up-regulated SA and HO production, resulting in enhanced disease resistance to . Silencing of in significantly decreased SA and HO accumulation, leading to the cotton more susceptibility. Moreover, combining the gene expression results from transgenic and silenced-GbEDS1 cotton, it indicated that could activate and expression. These findings not only broaden our knowledge about the biological role of , but also provide new insights into the defense mechanisms of against in cotton.

摘要

棉花是经济上最重要的作物之一，但大多数栽培品种缺乏足够的先天免疫力或对枯萎病的抗性。这导致产量和纤维品质都遭受严重损失。为了鉴定先天免疫的遗传资源并了解该作物中病原体防御的途径，我们在此聚焦于中央防御调节因子1（）的直系同源物。通过结合RACE策略筛选的全长cDNA文库获得了的全长cDNA。其开放阅读框长1848 bp，编码615个氨基酸残基。序列分析表明，GbEDS1包含一个保守的N端脂肪酶结构域和一个EDS1特异性的KNEDT基序。表达谱分析表明，该基因受以及水杨酸（SA）处理诱导。亚细胞定位分析显示，GbEDS1位于细胞质和细胞核中。在中过表达显著上调了SA和HO的产生，从而增强了对的抗病性。在中沉默显著降低了SA和HO的积累，导致棉花更易感病。此外，结合转基因和沉默GbEDS1棉花的基因表达结果表明，可激活和表达。这些发现不仅拓宽了我们对生物学作用的认识，也为棉花抗的防御机制提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/673d/5156716/cbc8d6d51ea9/fpls-07-01830-g011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/673d/5156716/0dd1b6dfc79e/fpls-07-01830-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/673d/5156716/93e7bc8cea46/fpls-07-01830-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/673d/5156716/126eb29b85aa/fpls-07-01830-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/673d/5156716/cbc8d6d51ea9/fpls-07-01830-g011.jpg

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