Shore E M, Guild G M
J Mol Biol. 1986 Jul 20;190(2):149-58. doi: 10.1016/0022-2836(86)90288-3.
The structure of the Drosophila melanogaster salivary gland secretion gene Sgs-5 has been determined by DNA sequence analysis of cloned genomic DNA. This developmentally and tissue-specific gene is a member of the third instar intermolt gene set and is under control of the insect molting hormone ecdysterone. RNA protection experiments show that the RNA coding region of Sgs-5 contains 769 nucleotides and is divided into three exons by two small introns. The protein-coding region appears to begin after a short untranslated RNA leader (33 nucleotides) and to result in a protein of 163 amino acids. The first 18 amino acids give the amino-terminal end the highly hydrophobic nature characteristic of a signal peptide.
通过对克隆的基因组DNA进行DNA序列分析，确定了黑腹果蝇唾液腺分泌基因Sgs-5的结构。这个在发育和组织上具有特异性的基因是三龄幼虫蜕皮间期基因集的成员，受昆虫蜕皮激素蜕皮甾酮的调控。RNA保护实验表明，Sgs-5的RNA编码区包含769个核苷酸，被两个小内含子分为三个外显子。蛋白质编码区似乎在一段短的非翻译RNA前导序列（33个核苷酸）之后开始，产生一个由163个氨基酸组成的蛋白质。前18个氨基酸使氨基末端具有信号肽特有的高度疏水性质。
