Frasch M, Saumweber H
Max Planck Institut für Entwicklungsbiologie Abt. 1, Tübingen, Federal Republic of Germany.
Chromosoma. 1989 Jan;97(4):272-81. doi: 10.1007/BF00371966.
Immunizing chromatin protein fractions from Drosophila melanogaster embryos, monoclonal antibodies have been generated against two nuclear proteins of different molecular weight. These proteins are present in a chromatin fraction of Drosophila Kc-cell nuclei and both proteins could be shown to cosediment with nucleosomes following separation on sucrose gradients. Early in development both proteins are located in the embryo cytoplasm. Later, at times when transcription starts at blastoderm, they become redistributed into the nuclei. On salivary gland chromosomes both proteins are detected in a series of developmentally active puffs. The number of sites where these antigens can be detected, as well as the qualitative properties of the antigens at these sites differ between both proteins.
通过免疫果蝇胚胎的染色质蛋白组分，已产生了针对两种不同分子量核蛋白的单克隆抗体。这些蛋白存在于果蝇Kc细胞核的染色质组分中，并且在蔗糖梯度分离后，这两种蛋白都可与核小体共沉降。在发育早期，这两种蛋白都位于胚胎细胞质中。后来，在胚盘开始转录时，它们重新分布到细胞核中。在唾液腺染色体上，在一系列发育活跃的胀泡中都检测到了这两种蛋白。这两种蛋白在可检测到这些抗原的位点数量以及这些位点抗原的定性特性方面存在差异。
