Lin Yan, Xiao Rong, Yin Lin-Zi, Wu Ping, Liu Yue-Xin, Li Chun, Lin Li-Mei, Liao Duan-Fang
Key Laboratory for Quality Evaluation of Bulk Herbs, College of Pharmacy, Hunan University of Chinese Medicine Changsha 410208, China.
College of Physics and Electronic, Central South University Changsha 410012, China.
Zhongguo Zhong Yao Za Zhi. 2019 Feb;44(3):509-517. doi: 10.19540/j.cnki.cjcmm.20181114.001.
Idiosyncratic hepatotoxicity of Polygonum multiflorum has attracted a great attention in the world. The most toxic part of idiosyncratic hepatotoxicity was screened by MTT assay and flow cytometry, which was the 50% ethanol elute by macroporous adsorptive resins from alcohol-extraction of P. multiflorum. The fingerprints were collected by HPLC from 50% ethanol elute of crude and processed P. multiflorum from different habitats, then 14 common peaks were determined. Spectrum-toxicity relationship was analyzed by rough set theory(RST). Two main chemical components were predicted for idiosyncratic hepatotoxicity, in which TSG was the greater contributor. Idiosyncratic hepatotoxicity of TSG was tested in vitro, and the results indicated that TSG was the most important constituent contributed to idiosyncratic hepatotoxicity of P. multiflorum. The study showed the discovery of the main chemical components for idiosyncratic hepatotoxicity, and RST was effective for analyzing the spectrum-toxicity relationship, which could be a new method used in the effective/toxic constituents field of traditional Chinese medicine.
何首乌的特异质性肝毒性已引起全球广泛关注。通过MTT法和流式细胞术筛选出特异质性肝毒性的最毒部位，即何首乌醇提取物经大孔吸附树脂洗脱得到的50%乙醇洗脱物。采用HPLC法收集不同产地生熟何首乌50%乙醇洗脱物的指纹图谱，共确定14个共有峰。运用粗糙集理论（RST）分析谱-毒关系。预测了何首乌特异质性肝毒性的两个主要化学成分，其中二苯乙烯苷（TSG）贡献较大。对TSG的特异质性肝毒性进行体外试验，结果表明TSG是何首乌特异质性肝毒性的最重要成分。该研究揭示了何首乌特异质性肝毒性的主要化学成分，且RST对分析谱-毒关系有效，可为中药有效/毒性成分领域提供一种新方法。
