Mou Xiong-Jun, Ming Hao, Xu Le-Si, Yue Ying-Ying, Chen Yu, Chen Xin, Liu Song-Lin
Hubei Key Laboratory of Resource and Chemistry of Chinese Medicine, Hubei University of Chinese Medicine Wuhan 430065, China.
Zhongguo Zhong Yao Za Zhi. 2019 Feb;44(3):526-534. doi: 10.19540/j.cnki.cjcmm.20181129.003.
To study the antidepressant effect of Shugan Hewei Tang on chronic stress depression model rats, and select the effective substance fractions. Several male SD rats were randomly divided into 17 groups: blank control group, model group, positive control group(fluoxetine), Shugan Hewei Tang high and low dose groups, 6 high and low dose groups of different substance fractions. After modeling for 3 weeks and administration for 1 week, the effective substance fractions were selected according to the body mass and behavioral performance of SD rats in each group; several neurotransmitters in hippocampus of rats were determined by LC-MS/MS method, including norepinephrine(NE), serotonin(5-HT), 5-indoleacetic acid(5-HIAA), r-aminobutyric acid(GABA), and glutamic acid(Glu). Behavioral results after modeling showed that as compared with the blank group, the body mass growth of the model group was significantly reduced(P<0.01); the sugar water consumption was reduced(P<0.01); the distance between the open field and the number of crossing the central area were also significantly reduced(P<0.01, P<0.01); the resting time was increased significantly(P<0.01); and the forced swimming time was significantly prolonged(P<0.01), indicating that the depression model was effective. After intragastric administration, as compared with the model group, the above detection indicators of volatile oils, total polysaccharides and terpenoid in Fluoxetine, Shugan Hewei Tang groups were all changed(P<0.05 or P<0.01). The levels of NE, 5-HT and GABA in the hippocampus of the model group were significantly lower than those in the blank group(P<0.01), and the levels of 5-HIAA and Glu were significantly increased(P<0.01). As compared with the model group, neurotransmitters of the treatment groups were changed obviously or significantly(P<0.05 or P<0.01). Shugan Hewei Tang showed obvious anti-depressant effects, and the volatile oils, total polysaccharides and terpenoids acted as the main active substances. The mechanism of anti-depression may be related to its regulation of various neurotransmitter levels in the hippocampus.
研究疏肝和胃汤对慢性应激抑郁模型大鼠的抗抑郁作用，并筛选有效物质部位。将若干雄性SD大鼠随机分为17组：空白对照组、模型组、阳性对照组（氟西汀）、疏肝和胃汤高、低剂量组、6个不同物质部位的高、低剂量组。造模3周并给药1周后，根据各组SD大鼠的体质量和行为学表现筛选有效物质部位；采用液相色谱-串联质谱法测定大鼠海马组织中几种神经递质，包括去甲肾上腺素（NE）、5-羟色胺（5-HT）、5-吲哚乙酸（5-HIAA）、γ-氨基丁酸（GABA）和谷氨酸（Glu）。造模后的行为学结果显示，与空白组相比，模型组体质量增长显著降低（P<0.01）；糖水消耗量降低（P<0.01）；旷场试验中穿越中央区域的距离和次数也显著减少（P<0.01，P<0.01）；静止时间显著增加（P<0.01）；强迫游泳时间显著延长（P<0.01），表明抑郁模型造模成功。灌胃给药后，与模型组相比，氟西汀组、疏肝和胃汤组挥发油、总多糖、萜类的上述检测指标均有变化（P<0.05或P<0.01）。模型组海马组织中NE、5-HT和GABA水平显著低于空白组（P<0.01），5-HIAA和Glu水平显著升高（P<0.01）。与模型组相比，各治疗组神经递质有明显或显著变化（P<0.05或P<0.01）。疏肝和胃汤具有明显的抗抑郁作用，挥发油、总多糖和萜类为主要活性物质。其抗抑郁机制可能与其调节海马组织中多种神经递质水平有关。
