Cao Mei-Qun, Chen De-Heng, Zhang Chun-Hu, Wu Zheng-Zhi
The 2nd Clinical Medical College of Jinan University/Shenzhen Geriatrics Research Institute, Shenzhen 518020, China.
Zhongguo Zhong Yao Za Zhi. 2013 May;38(10):1585-9.
To screen microRNAs with specific expression of in hippocampus of rats with chronic stress induced depression model, and observe the effect of traditional Chinese medicine Chaihu Shugan San on the expression of microRNA in hippocampus.
SD rats were randomly divided into 3 groups: the normal control group, the model control group and the Chaihu Shugan San group. The depression model was replicated by unpredictable chronic mild stress combined with separation. Behavioral changes of the rats were observed by Open-field test and sucrose solution consumption test, and the expression of microRNAs in hippocampus was assayed by microRNA micro-array.
Compared with the normal control group, there were 13 specific miRNAs in hippocampus in the model control group with the expression difference of more than 2 times. Among them, down-regulating miRNAs included miR298, miR-130b, miR-135a, miR-323, miR-503, miR-15b, miR-532, and miR-125a, and the up-regulation miRNAs included miR7a, miR-212, miR-124, miR-139, and miR-182. Among the 13 specific miRNAs, miR-125a and miR-182 recovered to normal after intervention with Chaihu Shugan San in the Chaihu Shugan San group.
This study preliminarily found that 13 specific miRNAs in hippocampus are related to depression. Among them, miR-125a and miR-182 recover to normal after intervention with Chaihu Shugan San, which may be the target points of the antidepressant effect of Chaihu Shugan San. We shall further analyze the target genes and their mechanisms.
筛选慢性应激诱导抑郁模型大鼠海马中具有特异性表达的微小RNA，并观察中药柴胡疏肝散对海马微小RNA表达的影响。
将SD大鼠随机分为3组：正常对照组、模型对照组和柴胡疏肝散组。采用不可预测的慢性轻度应激结合分离的方法复制抑郁模型。通过旷场试验和蔗糖溶液消耗试验观察大鼠的行为变化，采用微小RNA微阵列检测海马中微小RNA的表达。
与正常对照组相比，模型对照组海马中有13种特异性微小RNA，表达差异超过2倍。其中，下调的微小RNA包括miR298、miR-130b、miR-135a、miR-323、miR-503、miR-15b、miR-532和miR-125a，上调的微小RNA包括miR7a、miR-212、miR-124、miR-139和miR-182。在这13种特异性微小RNA中，柴胡疏肝散组经柴胡疏肝散干预后，miR-125a和miR-182恢复正常。
本研究初步发现海马中13种特异性微小RNA与抑郁症有关。其中，miR-125a和miR-182经柴胡疏肝散干预后恢复正常，可能是柴胡疏肝散抗抑郁作用的靶点。我们将进一步分析其靶基因及其机制。
