Huang Jing-Wen, Liu Juan, Zhou Jun-Hui, Yuan Yuan, Yang Quan, Huang Lu-Qi
School of Traditional Chinese Medicine,Guangdong Pharmaceutical University Guangzhou 510006,China National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
Zhongguo Zhong Yao Za Zhi. 2019 Mar;44(6):1151-1155. doi: 10.19540/j.cnki.cjcmm.20190129.014.
Armillaria gallica is a facultative parasitic fungus which is the only nutrient source of Gastrodia elata during its cultivation.Chitinase,as a glycosidic hydrolytic enzyme,plays an important role in the growth,development,stress tolerance and symbiotic signal transduction of A. gallica. There were 22 chitinase genes in A. gallica. Bioinformatics analysis of amino acid sequence of these chitinase genes revealed that 12 chitinase genes contained glycosidase 18 family( GH18) domain. Chitinase amino acid sequences of A. gallica,A. ostoyae,G. elata,Saccharomyces cerevisiae and Trichoderma harzianum were analyzed byclustering trees,so as to further predict the gene function of chitinase in A. gallica. Induction of A. gallica branching with strigolactone analogue GR24,high-throughput sequencing technology based on the induction of branch group( MHJ1),uninduced branch group( MHJ2) and blank control group( MHJ3) is used to detect the expression quantity,the transcription level data of 22 chitinase genes were obtained and the heat map was generated for expression pattern analysis. It was found that 8 genes may be involved in physiological processes such as A. gallica branching,cell wall degradation and remodeling. In this paper,the function of chitinase gene in A. gallica was just preliminarily analyzed and predicted.
蜜环菌是一种兼性寄生真菌，是天麻栽培过程中的唯一营养来源。几丁质酶作为一种糖苷水解酶，在蜜环菌的生长、发育、胁迫耐受性和共生信号转导中发挥着重要作用。蜜环菌中有22个几丁质酶基因。对这些几丁质酶基因的氨基酸序列进行生物信息学分析发现，有12个几丁质酶基因含有糖苷酶18家族（GH18）结构域。通过聚类树分析蜜环菌、奥氏蜜环菌、天麻、酿酒酵母和哈茨木霉的几丁质酶氨基酸序列，以进一步预测蜜环菌中几丁质酶的基因功能。用独脚金内酯类似物GR24诱导蜜环菌分枝，基于诱导分枝组（MHJ1）、未诱导分枝组（MHJ2）和空白对照组（MHJ3），利用高通量测序技术检测表达量，获得22个几丁质酶基因的转录水平数据并生成热图进行表达模式分析。发现有8个基因可能参与蜜环菌分枝、细胞壁降解和重塑等生理过程。本文仅对蜜环菌中几丁质酶基因的功能进行了初步分析和预测。
