Liang Yu-Ting, Jiang Chao, Zhou Jun-Hui, Hu Qi-Tiao, Yuan Yuan
State Key Laboratory of Dao-di Herbs Breeding Base,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
Zhongguo Zhong Yao Za Zhi. 2019 Sep;44(17):3622-3626. doi: 10.19540/j.cnki.cjcmm.20190701.102.
Armillaria gallica is a symbiotic fungus in the cultivation process of Gastrodia elata and Polyporus.The rhizomorph of A. gallica invades the stalk of the G. elata or the Sclerotium of the Polyporus,and is digested and utilized by the latter,becoming their important source of nutrition. Different nature of A. gallica affects the growth of G. elata and Polyporus. The authors collected A. gallica from 13 commercially available regions and screened two A. gallica,A and B,at the genetic and metabolic levels,in order to distinguish between the two A. gallica market. We have established convenient and effective DNA molecular identification method.By comparing the sequence differences between the A. gallica type A and type B invertase genes,PCR-RFLP primers were designed based on differential fragment. Primer ZTM.F/ZTM.R can amplified A. gallica type A and B,producing a band of about 304 bp in length. The restriction endonuclease EcoR V could recognize the difference sequence of A and B types of A. gallica. The type B was digested to form two fragments,thereby specifically identifying the A. gallica as type B. The established methods of PCR-RFLP is an accurate identification method for A. gallica. Therefore,in the cultivation process of G. elata and Polyporus,suitable strains can be selected according to different needs of variety,growth stage and ecological environment,and the yield and quality can be improved according to local conditions.
密环菌是天麻和猪苓栽培过程中的一种共生真菌。密环菌的菌索侵入天麻的茎或猪苓的菌核，并被后者消化利用，成为它们重要的营养来源。不同性质的密环菌影响天麻和猪苓的生长。作者从13个市售地区采集密环菌，并在基因和代谢水平上筛选出两种密环菌A和B，以区分两种密环菌市场。我们建立了便捷有效的DNA分子鉴定方法。通过比较密环菌A型和B型转化酶基因之间的序列差异，基于差异片段设计了PCR-RFLP引物。引物ZTM.F/ZTM.R可扩增密环菌A型和B型，产生一条约304bp长的条带。限制性内切酶EcoR V可识别密环菌A、B型的差异序列。B型被消化形成两个片段，从而特异性鉴定密环菌为B型。所建立的PCR-RFLP方法是一种准确鉴定密环菌的方法。因此，在天麻和猪苓的栽培过程中，可根据品种、生长阶段和生态环境的不同需求选择合适的菌株，并因地制宜提高产量和质量。
