Wang Chun-Xue, Liu Rui, Qian Da-Wei, Duan Jin-Ao
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine Nanjing 210023,China Jiangsu Key Laboratory for High Technology Research of TCM Formulae Nanjing 210023,China.
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine Nanjing 210023,China Jiangsu Key Laboratory for High Technology Research of TCM Formulae Nanjing 210023,China Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics Nanjing 210023,China.
Zhongguo Zhong Yao Za Zhi. 2019 Mar;44(6):1216-1219. doi: 10.19540/j.cnki.cjcmm.20181226.015.
This present study is to detect the content of free thiols(-SH) in the horn derived traditional Chinese medicines( TCMs) from different animals and different regions by using fluorescence derivatization method. TCEP was used as a disulfide bond reducing agent,while SBD-F as a derivatization reagent. Fluorescent spectrophotometry was used to determine the content of-SH,and the maximum excitation wavelength and emission wavelength were set as 375 and 510 nm,respectively. As a result,under the optimized condition,the extraction of Caprae Hircus Cornu showed the highest free-SH concentration,followed by Bovis Grunniens Cornu,Bubali Cornu,and Elaphuri Davidiani Cornu. In the present study,we point out that the-SH-contained components might be the most important material basis in animal horn derived TCMs. With good accurate,sensitive and rapid properties,the present method can provide reference basis for the quality evaluation of animal horn derived TCMs and guides for the investigation on effective material basis.
本研究采用荧光衍生化法检测不同动物、不同产地的角类中药中游离巯基（-SH）的含量。以三（2-羧乙基）膦（TCEP）作为二硫键还原剂，以SBD-F作为衍生化试剂。采用荧光分光光度法测定-SH含量，最大激发波长和发射波长分别设定为375nm和510nm。结果显示，在优化条件下，山羊角提取物中游离-SH浓度最高，其次是牦牛角、水牛角和鹿角。本研究指出，含-SH的成分可能是角类中药中最重要的物质基础。该方法具有良好的准确性、灵敏性和快速性，可为角类中药的质量评价提供参考依据，并为有效物质基础的研究提供指导。
