Liu Rui, Zhao Ming, Liu Xiao, Yang Chao, Chen Sheng-Jun, Duan Jin-Ao
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine Nanjing 210023, China School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics, Nanjing University of Chinese Medicine Nanjing 210023, China.
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine Nanjing 210023, China School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
Zhongguo Zhong Yao Za Zhi. 2022 Mar;47(5):1279-1285. doi: 10.19540/j.cnki.cjcmm.20211206.201.
This paper explored the specific peptides from Bubali Cornu by ultra-performance liquid chromatography-tandem mass spectrometry and based on mathematics set theory. Following the profile analysis of peptides from Bubali Cornu, Bovis Grunniens Cornu, Caprae Hircus Cornu, and Suis Cornu by nano LC-LTQ-Obitrap-MS after digestion with trypsin, the relationship of peptide composition among different samples was analyzed using the mathematics set theory. The ones that existed only in the Bubali Cornu set rather than in any other set were considered as the specific peptides of Bubali Cornu. The further bioinformatic analysis revealed four specific peptides from Bubali Cornu, whose specificity was verified by UPLC-QQQ-MS. The results showed that these four peptides could be used for distinguishing Bubali Cornu from Caprae Hircus Cornu and Suis Cornu. This study has provided a rapid and simple method for seeking the specific peptides in animal medicines, which can be utilized for quality evaluation of animal medicines, thus making them authenticable and traceable.
本文采用超高效液相色谱-串联质谱法并基于数学集合论，对水牛角中的特定肽段进行了探索。在用胰蛋白酶消化后，通过纳升液相色谱-线性离子阱-轨道阱质谱仪对水牛角、牦牛角、山羊角和猪角中的肽段进行谱图分析，然后运用数学集合论分析不同样品间肽段组成的关系。仅存在于水牛角集合而非其他任何集合中的肽段被视为水牛角的特定肽段。进一步的生物信息学分析揭示了水牛角中的四种特定肽段，其特异性通过超高效液相色谱-三重四极杆质谱仪得到验证。结果表明，这四种肽段可用于区分水牛角与山羊角和猪角。本研究为寻找动物药中的特定肽段提供了一种快速简便的方法，可用于动物药的质量评价，从而使其具有可鉴定性和可追溯性。
