Liu Rui, Zhu Zhen-Hua, Wu Jia, Qian Da-Wei, Duan Jin-Ao
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics, Nanjing 210023, China.
Zhongguo Zhong Yao Za Zhi. 2018 Aug;43(16):3329-3334. doi: 10.19540/j.cnki.cjcmm.20180511.001.
SDS-PAGE and LC-MS/MS were used to identify proteins in Saigae Tataricae Cornu (SAH) and Caprae Hircus Cornu (GH). Trypsin digestion peptides from SAH and GH were obtained by in-gel digestion, after which nano LC-LTQ/Orbitrap MS was used to identify the proteins in SAH and GH. As a result, in total 101 proteins and 140 proteins were identified form SAH and GH, respectively. There were 43 keratins (KRTs) and keratin-associated proteins (KAPs) identified, which account for 42.6% of the 101 proteins in SAH. The proportion of KRTs and KAPs in GH was 37.1%. The comparison between SAH and GH showed that the main common components in SAH and GH were structural molecule activity proteins, such as KRTs and KAPs. In the present study, we provide determination method and experimental data for investigating the material basis of SAH and GH, guiding the investigation on effective material basis and quality standard of animal horn derived TCMs.
采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和液相色谱-串联质谱（LC-MS/MS）对羚羊角（SAH）和山羊角（GH）中的蛋白质进行鉴定。通过凝胶内消化获得SAH和GH的胰蛋白酶消化肽段，之后采用纳升液相色谱-线性离子阱/轨道阱质谱（nano LC-LTQ/Orbitrap MS）对SAH和GH中的蛋白质进行鉴定。结果，分别从SAH和GH中鉴定出101种和140种蛋白质。共鉴定出43种角蛋白（KRTs）和角蛋白相关蛋白（KAPs），占SAH中101种蛋白质的42.6%。KRTs和KAPs在GH中的比例为37.1%。SAH和GH的比较表明，SAH和GH中的主要共同成分是结构分子活性蛋白，如KRTs和KAPs。在本研究中，我们为研究SAH和GH的物质基础提供了测定方法和实验数据，为指导动物角类中药有效物质基础及质量标准的研究提供依据。
