Akiyoshi T, Wada T, Arinaga S, Koba F, Tsuji H
Jpn J Surg. 1986 Sep;16(5):323-9. doi: 10.1007/BF02470554.
Utilizing a clonogenic assay, the effects of hyperthermia and selected chemotherapeutic drugs on growth of cells from malignant effusions were studied. Fourteen of 25 samples obtained from 25 patients with various carcinomas formed at least 30 colonies per plate. Exposure of the cells to heat at 42 degrees C for 1 hr before the plating slightly inhibited the colony growth. The drugs, adriamycin (AM) and mitomycin C (MMC), were tested at 3 different concentrations. When the cells were treated with these two drugs for 1 hr at 42 degrees C, the percent of surviving colonies was significantly decreased, as compared to findings at 37 degrees C, in both groups, at 3 different concentrations. The combination of drugs and hyperthermia appeared to function synergistically in one-third of such cases. These results suggest that cells from malignant effusions in patients with various carcinomas were more sensitive to AM or MMC, under condition of a higher temperature (42 degrees C).
利用克隆形成试验，研究了热疗和选定的化疗药物对恶性积液细胞生长的影响。从25例患有各种癌症的患者中获取的25个样本中，有14个样本每平板至少形成30个集落。在接种前将细胞在42℃加热1小时，略微抑制了集落生长。对阿霉素（AM）和丝裂霉素C（MMC）这两种药物进行了3种不同浓度的测试。当细胞在42℃用这两种药物处理1小时时，与在37℃时的结果相比，在两组的3种不同浓度下，存活集落的百分比均显著降低。在三分之一的此类病例中，药物与热疗的联合似乎具有协同作用。这些结果表明，在较高温度（42℃）条件下，患有各种癌症患者的恶性积液细胞对AM或MMC更敏感。
