Rofstad E K, Brustad T
Cancer. 1982 Oct 1;50(7):1304-8. doi: 10.1002/1097-0142(19821001)50:7<1304::aid-cncr2820500715>3.0.co;2-h.
Human melanoma cells were exposed to clinically acceptable hyperthermia (42.5 degrees C) either as solid tumors in athymic nude mice or suspended in culture medium. Single cell survival was in both cases assayed in vitro in soft agar. The response to heat varied considerably among the five melanomas studied. The D0-values ranged from 21 to 590 min when the cells were heated in vitro. The response to heat following treatment in vivo was for a given melanoma larger than that following treatment in vitro. However, cells which were resistant to heat treatment in vitro, were also resistant to treatment in vivo, and those which were sensitive in vitro were also sensitive in vivo.
将人黑色素瘤细胞暴露于临床上可接受的热疗温度（42.5摄氏度）下，要么是在无胸腺裸鼠体内形成实体瘤，要么是悬浮于培养基中。两种情况下的单细胞存活率均在体外软琼脂中进行测定。在所研究的五种黑色素瘤中，对热的反应差异很大。当细胞在体外加热时，D0值范围为21至590分钟。对于给定的黑色素瘤，体内治疗后的热反应大于体外治疗后的热反应。然而，在体外对热处理有抗性的细胞，在体内对治疗也有抗性，而在体外敏感的细胞在体内也敏感。
