Chung Yoon Hee, Park Tae Kwang, Yim Sung Hyuk, Lee Jong Hyuk, Bang Joon Seok, Shin Yong Kyoo, Bae Jinhyung, Sim Sang Soo, Hwang Kwang Woo, Shin Chang Yell, Im Wi Joon, Khin Phyu Phyu, Lee Jaehwi, Min Young Sil, Jeong Ji Hoon, Sohn Uy Dong
1 College of Medicine, Chung-Ang University, Seoul, Korea.
2 College of Life and Health Science, Hoseo University, Asan, Korea.
J Med Food. 2019 Apr;22(4):355-364. doi: 10.1089/jmf.2018.4218.
Our study aimed to investigate the effects of the polysaccharide-rich extract of (PEP) against water immersion restraint (WIR) stress and forced swimming-induced fatigue. Exposure to WIR stress significantly increased the ulcer index, bleeding score, the weight of the adrenal gland, blood glucose concentrations, total cholesterol, cortisol, and creatine kinase (CK). The weight of the spleen decreased significantly. In addition, myeloperoxidase (MPO) and thiobarbituric acid-reactive substance (TBARS) were significantly upregulated by WIR stress. The antioxidative factors such as glutathione (GSH) and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in the stomach were decreased by WIR stress. Alterations induced by WIR stress were effectively reversed by pretreatment with PEP. The swimming endurance capacity of mice was significantly prolonged by the oral administration of PEP. Swimming-induced fatigue significantly reduced the body weight; however, the injection of PEP inhibited the decrease of body weight. The PEP-treated group had significantly lower CK levels in plasma, an indicator of muscle damage. These results indicated that PEP has anti-stress and anti-fatigue effects, which are mediated by suppressing the hyperactivation of the hypothalamus-pituitary-adrenal axis, and antagonism of the oxidative damages induced by WIR stress and prolonged swimming times.
我们的研究旨在探究[具体名称]富含多糖的提取物（PEP）对水浸束缚（WIR）应激和强迫游泳诱导疲劳的影响。暴露于WIR应激会显著增加溃疡指数、出血评分、肾上腺重量、血糖浓度、总胆固醇、皮质醇和肌酸激酶（CK）。脾脏重量显著降低。此外，WIR应激会显著上调髓过氧化物酶（MPO）和硫代巴比妥酸反应性物质（TBARS）。胃中的抗氧化因子如谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）会因WIR应激而降低。WIR应激引起的改变通过PEP预处理得到有效逆转。口服PEP可显著延长小鼠的游泳耐力。游泳诱导的疲劳显著降低体重；然而，注射PEP可抑制体重下降。PEP处理组血浆中的CK水平显著较低，CK是肌肉损伤的一个指标。这些结果表明，PEP具有抗应激和抗疲劳作用，其作用机制是抑制下丘脑-垂体-肾上腺轴的过度激活，以及对抗WIR应激和延长游泳时间诱导的氧化损伤。
