Engineering Research Centre of Bioprocess of Ministry of Education, Hefei University of Technology, No 193 Tunxi Road, Hefei 230009, People's Republic of China; School of Food and Biological Engineering, Hefei University of Technology, No 193 Tunxi Road, Hefei 230009, People's Republic of China.
Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences, Guangzhou 510610, People's Republic of China; Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs, Guangzhou 510610, People's Republic of China; Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, People's Republic of China.
Int J Biol Macromol. 2021 Apr 1;175:235-241. doi: 10.1016/j.ijbiomac.2021.01.200. Epub 2021 Feb 3.
In the present study, we explored the anti-fatigue activity and its potential mechanism of a purified Polygonatum cyrtonema polysaccharide (PCP) on mice using weight-loaded swimming test. Results showed that PCP remarkably prolonged the exhaustive swimming time of mice when compared with normal control group. Meanwhile, PCP decreased serum levels of lactic acid (LA), blood uric nitrogen (BUN), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA), and increased the contents of liver glycogen, muscle glycogen and muscle ATP. These results revealed that PCP had good anti-fatigue ability. The histomorphologic analysis showed that PCP increased the cross-section area of the muscle fibers. Furthermore, PCP significantly enhanced the protein levels of bone morphogenetic protein-2 (BMP-2), phosphor-Smad1, Runt-related transcription factor 2 (Runx2) and osteocalcin (OC) in skeleton. Similar variation was also observed in the expression of osteocalcin signaling mediators of phosphorylated cAMP-response element binding protein (p-CREB) and phosphorylated hormone-sensitive lipase (p-HSL) in skeletal muscle. These results suggested that PCP resisted fatigue possibly via regulating osteocalcin signaling.
在本研究中，我们使用负重游泳试验探讨了纯化黄精多糖（PCP）对小鼠的抗疲劳活性及其潜在机制。结果表明，与正常对照组相比，PCP 显著延长了小鼠的力竭游泳时间。同时，PCP 降低了血清乳酸（LA）、血尿素氮（BUN）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和丙二醛（MDA）的水平，增加了肝糖原、肌肉糖原和肌肉 ATP 的含量。这些结果表明 PCP 具有良好的抗疲劳能力。组织形态学分析表明，PCP 增加了肌纤维的横截面积。此外，PCP 还显著增强了骨骼中骨形态发生蛋白 2（BMP-2）、磷酸化 Smad1、Runt 相关转录因子 2（Runx2）和骨钙素（OC）的蛋白水平。在骨骼肌中，骨钙素信号转导介质磷酸化 cAMP 反应元件结合蛋白（p-CREB）和磷酸化激素敏感脂肪酶（p-HSL）的表达也观察到类似的变化。这些结果表明，PCP 通过调节骨钙素信号来抵抗疲劳。
