Hebei University of Science and Technology, Hebei, China.
J Sci Food Agric. 2021 Jun;101(8):3132-3139. doi: 10.1002/jsfa.10942. Epub 2020 Dec 1.
To estimate the anti-fatigue and antioxidative effects of water and alcohol extracts from Diaphragma juglandis (DJ), H O -treated HepG2 cells were used as an in vitro model to determine the total antioxidant capacities of these two extracts, and behavioral tests on mice and biochemical assay were performed via in vivo experiments.
The results indicate that both extracts possess remarkable HepG2 protective capacities and were capable of scavenging 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) similar to vitamin C. Furthermore, they could significantly prolong the bar climbing time and force swimming time, as well as decrease the serum urea nitrogen and increase the lactate dehydrogenase level and glycogen content. These extracts could also improve the activities of total antioxidant capacity, malondialdehyde, superoxide dismutase, catalase and glutathione peroxidase.
In conclusion, both water and alcohol extracts from DJ showed good performance with respect to anti-fatigue and could be a potential antioxidant additive in the field of functional foods. © 2020 Society of Chemical Industry.
为了评估来自黄杞（DJ）的水提物和醇提物的抗疲劳和抗氧化作用，采用 H 2 O 2 处理的 HepG2 细胞作为体外模型来确定这两种提取物的总抗氧化能力，并通过体内实验进行了小鼠行为测试和生化测定。
结果表明，这两种提取物均具有显著的 HepG2 保护能力，并且能够清除 2,2-二苯基-1-苦基肼基（2,2-diphenyl-1-picrylhydrazyl）和 2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)），类似于维生素 C。此外，它们还能显著延长攀爬时间和游泳时间，并降低血清尿素氮，同时增加乳酸脱氢酶水平和糖原含量。这些提取物还可以提高总抗氧化能力、丙二醛、超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性。
综上所述，DJ 的水提物和醇提物在抗疲劳方面表现良好，可能成为功能性食品领域中潜在的抗氧化添加剂。© 2020 英国化学学会。
