Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland.
Department of Animal Reproduction with a Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-719 Olsztyn, Poland.
Int J Food Microbiol. 2019 Jul 2;300:8-13. doi: 10.1016/j.ijfoodmicro.2019.04.004. Epub 2019 Apr 10.
The aim of the study was to investigate the presence of Shiga toxin-producing Escherichia coli (STEC) in the wild boar population of north-eastern Poland, and to evaluate the potential health risk associated with wild boars carrying STEC/AE-STEC strains. In Poland, the African Swine Fever (ASF) virus has been a growing problem in domestic pigs and wild boars, one of the main reservoirs of the virus, because of this hunters, veterinary practitioners and foresters thus face a greater risk of coming into contact with animals. Rectal swabs samples were obtained from 152 wild boars hunter-harvested in the 2017/2018 season (autumn-winter) in north-eastern Poland. The samples were enrichment in modified buffered peptone water. Polymerase chain reaction (PCR) assays were conducted to determine the virulence profile of stx1, stx2 and eae and aggR genes, identify subtypes of stx1 and stx2 genes, and perform O and H serotyping. STEC/AE-STEC virulence genes were detected in 43 isolates (28.29%): STEC in 17 isolates (11.18%) and AE-STEC in 26 isolates (17.11%), respectively. None of the tested isolates carried the aggR gene. The most dangerous AE-STEC virulence profile associated with HUS was found in 2 isolates (1.32%): stx1NS/stx2a/d/eae serotype ONT:H7 and stx2a/eae serotype O146:H7. Six of the 152 tested samples belonged to serogroup O157 (3.95%), including one AE-STEC isolate with virulence profile stx2g/eae and five EPEC isolates. The results of this study suggest that wild boars in north-eastern Poland can carry STEC/AE-STEC strains that are potentially pathogenic for humans. This is the first report documenting the virulence of STEC and AE-STEC isolates from wild boars in Poland.
本研究旨在调查波兰东北部野猪群体中是否存在产志贺毒素大肠杆菌（STEC），并评估携带 STEC/AE-STEC 菌株的野猪可能带来的健康风险。在波兰，非洲猪瘟（ASF）病毒在国内猪和野猪中已成为一个日益严重的问题，由于野猪是该病毒的主要宿主之一，因此猎人、兽医和林务员接触到携带病毒的动物的风险更大。2017/2018 季节（秋冬），从波兰东北部的 152 头野猪猎人收获的直肠拭子样本中采集了样本。样本在改良缓冲蛋白胨水中进行富集。进行聚合酶链反应（PCR）检测，以确定stx1、stx2 和 eae 和 aggR 基因的毒力谱，鉴定 stx1 和 stx2 基因的亚型，并进行 O 和 H 血清型鉴定。在 43 株分离株中检测到 STEC/AE-STEC 毒力基因（28.29%）：17 株（11.18%）为 STEC，26 株（17.11%）为 AE-STEC。未检测到携带 aggR 基因的分离株。与 HUS 相关的最危险的 AE-STEC 毒力谱在 2 株分离株中发现（1.32%）：stx1NS/stx2a/d/eae 血清型 ONT：H7 和 stx2a/eae 血清型 O146：H7。在 152 个测试样本中，有 6 个属于 O157 血清群（3.95%），包括 1 个携带毒力谱 stx2g/eae 的 AE-STEC 分离株和 5 个 EPEC 分离株。本研究结果表明，波兰东北部的野猪可能携带对人类具有潜在致病性的 STEC/AE-STEC 菌株。这是波兰首次记录来自野猪的 STEC 和 AE-STEC 分离株的毒力。
