Bertelloni Fabrizio, Cilia Giovanni, Bogi Samantha, Ebani Valentina Virginia, Turini Luca, Nuvoloni Roberta, Cerri Domenico, Fratini Filippo, Turchi Barbara
Department of Veterinary Science, University of Pisa, 56126 Pisa, Italy.
Animals (Basel). 2020 Apr 24;10(4):744. doi: 10.3390/ani10040744.
Wild boar are among the most widespread wild mammals in Europe. Although this species can act as a reservoir for different pathogens, data about its role as a carrier of pathogenic and antimicrobial-resistant are still scarce. The aim of this study was to evaluate the occurrence of antimicrobial-resistant and pathogenic in wild boar in the Tuscany region of Italy. During the hunting season of 2018-2019, was isolated from 175 of 200 animals and subjected to antimicrobial resistance tests and PCR for detection of resistance and virulence factor genes. The highest resistance rates were against cephalothin (94.3%), amoxicillin-clavulanic acid (87.4%), ampicillin (68.6%), and tetracycline (44.6%). The most detected resistance genes were (54.3%), (38.9%), (30.9%), and (24.6%). Concerning genes encoding virulence factors, 55 of 175 isolates (31.4%) were negative for all tested genes. The most detected genes were (47.4%), (29.1%), (24.6%), (17.1%), and (11.4%). was classified as Shiga toxin-producing (STEC) (21.7%), enterohemorrhagic (EHEC) (6.3%), enteroaggregative (EAEC) (5.1%), and atypical enteropathogenic (aEPEC) (3.4%). Enterotoxigenic (ETEC), enteroinvasive (EIEC), and typical enteropathogenic (tEPEC) were not detected. Our results show that wild boars could carry pathogenic and antimicrobial-resistant , representing a possible reservoir of domestic animal and human pathogens.
野猪是欧洲分布最广的野生哺乳动物之一。尽管该物种可作为多种病原体的宿主，但关于其作为致病和抗菌耐药菌携带者的作用的数据仍然匮乏。本研究的目的是评估意大利托斯卡纳地区野猪中抗菌耐药菌和致病菌的出现情况。在2018 - 2019年狩猎季节，从200只动物中的175只分离出[具体细菌名称未给出]，并进行抗菌药物耐药性测试和PCR检测，以检测耐药和毒力因子基因。最高耐药率针对头孢噻吩（94.3%）、阿莫西林 - 克拉维酸（87.4%）、氨苄西林（68.6%）和四环素（44.6%）。检测到的最常见耐药基因是[具体基因名称未给出]（54.3%）、[具体基因名称未给出]（38.9%）、[具体基因名称未给出]（30.9%）和[具体基因名称未给出]（24.6%）。关于编码毒力因子的基因，175株分离株中有55株（31.4%）对所有测试基因均为阴性。检测到的最常见基因是[具体基因名称未给出]（47.4%）、[具体基因名称未给出]（29.1%）、[具体基因名称未给出]（24.6%）、[具体基因名称未给出]（17.1%）和[具体基因名称未给出]（11.4%）。[具体细菌名称未给出]被分类为产志贺毒素大肠杆菌（STEC）（21.7%）、肠出血性大肠杆菌（EHEC）（6.3%）、肠聚集性大肠杆菌（EAEC）（5.1%）和非典型肠致病性大肠杆菌（aEPEC）（3.4%）。未检测到产肠毒素大肠杆菌（ETEC）、肠侵袭性大肠杆菌（EIEC）和典型肠致病性大肠杆菌（tEPEC）。我们的结果表明，野猪可能携带致病和抗菌耐药的[具体细菌名称未给出]，是家畜和人类病原体的一个潜在宿主。
