Szczerba-Turek Anna, Chierchia Filomena, Socha Piotr, Szweda Wojciech
Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland.
Department of Animal Reproduction with a Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-719 Olsztyn, Poland.
Animals (Basel). 2023 Mar 1;13(5):901. doi: 10.3390/ani13050901.
Wildlife can harbour Shiga toxin-producing (STEC). In the present study, STEC in faecal samples from red deer ( = 106) and roe deer ( = 95) were characterised. All isolates were non-O157 strains. In red deer, STEC were detected in 17.9% ( = 19) of the isolates, and the / virulence profile was detected in two isolates (10.5%). One STEC strain harboured (5.3%) and eighteen STEC strains harboured (94.7%). The most prevalent subtypes were ( = 12; 66.7%), ( = 3; 16.7%), and ( = 2; 11.1%). One isolate could not be subtyped (NS) with the applied primers (5.6%). The most widely identified serotypes were O146:H28 ( = 4; 21%), O146:HNM ( = 2; 10.5%), O103:H7 ( = 1; 5.3%), O103:H21 ( = 1; 5.3%), and O45:HNM ( = 1; 5.3%). In roe deer, STEC were detected in 16.8% ( = 16) of the isolates, and the / virulence profile was detected in one isolate (6.3%). Two STEC strains harboured (12.5%), one strain harboured / (6.3%), and thirteen strains harboured (81.3%). The most common subtypes were ( = 8; 61.5%), ( = 2; 15.4%), non-typeable subtypes (NS) ( = 2; 15.4%), and ( = 1; 7.7%). Serotype O146:H28 ( = 5; 31.3%) was identified. The study demonstrated that the zoonotic potential of STEC strains isolated from wildlife faeces should be monitored in the context of the 'One Health' approach which links human health with animal and environmental health.
野生动物可能携带产志贺毒素大肠杆菌（STEC）。在本研究中，对马鹿（n = 106）和狍（n = 95）粪便样本中的STEC进行了特征分析。所有分离株均为非O157菌株。在马鹿中，17.9%（n = 19）的分离株检测到STEC，两个分离株（10.5%）检测到stx1/stx2毒力谱。一株STEC菌株携带stx1（5.3%），十八株STEC菌株携带stx2（94.7%）。最常见的stx2亚型是stx2d（n = 12；66.7%）、stx2c（n = 3；16.7%）和stx2e（n = 2；11.1%）。一个分离株使用所应用的引物无法分型（NS）（5.6%）。最广泛鉴定的血清型是O146:H28（n = 4；21%）、O146:HNM（n = 2；10.5%）、O103:H7（n = 1；5.3%）、O103:H21（n = 1；5.3%）和O45:HNM（n = 1；5.3%）。在狍中，16.8%（n = 16）的分离株检测到STEC，一个分离株（6.3%）检测到stx1/stx2毒力谱。两株STEC菌株携带stx2（12.5%），一株携带stx1/stx2（6.3%），十三株携带stx2（81.3%）。最常见的亚型是stx2d（n = 8；61.5%）、stx2c（n = 2；15.4%）、不可分型亚型（NS）（n = 2；15.4%）和stx2e（n = 1；7.7%）。鉴定出血清型O146:H28（n = 5；31.3%）。该研究表明，应在将人类健康与动物和环境健康联系起来的“同一健康”方法的背景下，监测从野生动物粪便中分离出的STEC菌株的人畜共患病潜力。
