在酿酒酵母中，DNA结合对于调节蛋白的核定位并不充分。
DNA binding is not sufficient for nuclear localization of regulatory proteins in Saccharomyces cerevisiae.
作者信息
Silver P A, Brent R, Ptashne M
出版信息
Mol Cell Biol. 1986 Dec;6(12):4763-6. doi: 10.1128/mcb.6.12.4763-4766.1986.
Abstract
We showed by immunofluorescence that the procaryotic DNA-binding protein LexA and a chimeric protein that contains the DNA-binding portion of LexA (amino acids 1 to 87) and a large portion (amino acids 74 to 881) of the Saccharomyces cerevisiae positive regulatory GAL4 protein (GAL4 gene product) are not preferentially localized in the nucleus in S. cerevisiae.
摘要
我们通过免疫荧光显示，原核生物DNA结合蛋白LexA以及一种嵌合蛋白（该嵌合蛋白包含LexA的DNA结合部分（氨基酸1至87）和酿酒酵母阳性调节蛋白GAL4的一大部分（氨基酸74至881））在酿酒酵母中并非优先定位于细胞核。
Zotero 插件