State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences (ICR, CAAS), Anyang 455000, China.
Jaramogi Oginga Odinga University of Science and Technology (JOOUST), School of Biological and Physical Sciences (SPBS), P.O BOX 210-40600, Bondo, Kenya.
Int J Mol Sci. 2019 Apr 15;20(8):1863. doi: 10.3390/ijms20081863.
Reactive oxygen species (ROS) are important molecules in the plant, which are involved in many biological processes, including fiber development and adaptation to abiotic stress in cotton. We carried out transcription analysis to determine the evolution of the ROS genes and analyzed their expression levels in various tissues of cotton plant under abiotic stress conditions. There were 515, 260, and 261 genes of ROS network that were identified in (AD₁ genome), (A genome), and (D genome), respectively. The network genes were found to be distributed in all the cotton chromosomes, but with a tendency of aggregating on either the lower or upper arms of the chromosomes. Moreover, all the cotton network genes were grouped into 17 families as per the phylogenetic tress analysis. A total of 243 gene pairs were orthologous in and . There were 240 gene pairs that were orthologous in , , and . The synonymous substitution value () peaks of orthologous gene pairs between the At subgenome and the A progenitor genome (), D subgenome and D progenitor genome () were 0.004 and 0.015, respectively. The peaks of ROS network orthologous gene pairs between the two progenitor genomes (A and D genomes) and two subgenomes (At and Dt subgenome) were 0.045. The majority of / value of orthologous gene pairs between the A, D genomes and two subgenomes of TM-1 were lower than 1.0. RNA seq. analysis and RT-qPCR validation, showed that, CSD1,2,3,5,6; FSD1,2; MSD1,2; APX3,11; FRO5.6; and RBOH6 played a major role in fiber development while CSD1, APX1, APX2, MDAR1, GPX4-6-7, FER2, RBOH6, RBOH11, and FRO5 were integral for enhancing salt stress in cotton. ROS network-mediated signal pathway enhances the mechanism of fiber development and regulation of abiotic stress in Gossypium. This study will enhance the understanding of ROS network and form the basic foundation in exploring the mechanism of ROS network-involving the fiber development and regulation of abiotic stress in cotton.
活性氧（ROS）是植物中的重要分子，参与许多生物过程，包括纤维发育和对棉花非生物胁迫的适应。我们进行了转录分析，以确定 ROS 基因的进化，并分析了它们在棉花植物各种组织中在非生物胁迫条件下的表达水平。在 (AD₁基因组)、 (A 基因组) 和 (D 基因组) 中分别鉴定出 515、260 和 261 个 ROS 网络基因。ROS 网络基因被发现分布在棉花的所有染色体上，但有聚集在染色体的长臂或短臂上的趋势。此外，根据系统发育树分析，所有棉花 ROS 网络基因被分为 17 个家族。在 和 中，共有 243 对基因是直系同源的。在 、 和 中，有 240 对基因是直系同源的。在 At 亚基因组和 A 祖先基因组（）、D 亚基因组和 D 祖先基因组（）之间的直系同源基因对中，同义替代值（）的峰值分别为 0.004 和 0.015。在两个祖先基因组（A 和 D 基因组）和两个亚基因组（At 和 Dt 亚基因组）之间的 ROS 网络直系同源基因对中， 的峰值为 0.045。TM-1 的 A、D 基因组和两个亚基因组之间的直系同源基因对的 / 值大多数低于 1.0。RNA seq. 分析和 RT-qPCR 验证表明，CSD1、2、3、5、6；FSD1、2；MSD1、2；APX3、11；FRO5.6；和 RBOH6 在纤维发育中起主要作用，而 CSD1、APX1、APX2、MDAR1、GPX4-6-7、FER2、RBOH6、RBOH11 和 FRO5 对增强棉花的耐盐性至关重要。ROS 网络介导的信号通路增强了纤维发育和 Gossypium 非生物胁迫调节的机制。本研究将增强对 ROS 网络的理解，并为探索 ROS 网络参与棉花纤维发育和非生物胁迫调节的机制奠定基础。
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