Nowak J A, Jain N K, Stinson M W, Merrick J M
Mol Immunol. 1986 Jul;23(7):693-700. doi: 10.1016/0161-5890(86)90079-9.
A human Hanganutziu-Deicher (H-D) serum reacted with N-glycolylneuraminic acid (NeuGc)-containing gangliosides and glycoproteins isolated from bovine erythrocyte membranes. Three populations of H-D antibodies were identified in the human H-D serum. One population very likely recognized the NeuGc-Gal sequence; a second population appears to recognize additional sugars in the oligosaccharide sequence, e.g. NeuGc-Gal-GlcNAc; while a third population may also recognize polypeptide determinants in addition to the NeuGc-Gal-GlcNAc sequence. The H-D serum distinguished two high mol. wt glycoproteins (HMGP I and II) present in crude extracts of bovine erythrocyte membranes. These glycoproteins were separated by repetitive fractionation on Sephacryl S-1000 in the presence of urea and their composition determined.
一种人源汉努齐乌-戴歇尔（H-D）血清与从牛红细胞膜分离得到的含N-羟乙酰神经氨酸（NeuGc）的神经节苷脂和糖蛋白发生反应。在人源H-D血清中鉴定出三类H-D抗体。一类抗体很可能识别NeuGc-Gal序列；另一类抗体似乎识别寡糖序列中的其他糖类，例如NeuGc-Gal-GlcNAc；而第三类抗体除了识别NeuGc-Gal-GlcNAc序列外，可能还识别多肽决定簇。H-D血清区分了牛红细胞膜粗提物中存在的两种高分子量糖蛋白（HMGP I和II）。这些糖蛋白在尿素存在的情况下通过在Sephacryl S-1000上的重复分级分离进行分离，并确定了它们的组成。
